日本血吸虫病合并HBV感染患者肝纤维化4项指标的检测评估  

Evaluation on four biomarkers for liver fibrosis in schistosomiasis japonicum cases co-infected with hepatitis B virus

作　　者：朱剑君[1] 黄令霞[1] 汪军[1] 赵雅琴[1] 

机构地区：[1]湖南省血吸虫病防治所临床研究室,岳阳市414000

出　　处：《热带病与寄生虫学》2007年第4期208-210,共3页Journal of Tropical Diseases and Parasitology

基　　金：湖南省卫生厅重点课题基金资助(编号:A2004-2006)

摘　　要：目的探讨晚期和慢性血吸虫病合并HBV(乙肝病毒)感染患者血清肝纤维化4项指标,即透明质酸(HA)、Ⅲ型前胶原(CIII)、Ⅳ型胶原(IV.C)、层粘蛋白(LN)含量的变化。方法用放射免疫法测定125例晚期血吸虫病中26例合并"大三阳"、48例合并"小三阳",51例未合并HBV感染;145例慢性血吸虫病患者中25例合并HBV"大三阳"、35例"小三阳"、85例未合并HBV感染者的上述4项指标;对照组50例正常人血清肝纤维化4项指标的含量。HBV标志物采用ELISA一步法检测,并对其结果进行比较分析。结果晚血患者血清肝纤维化4项指标含量明显高于对照组(P<0.01);晚血并HBV"大三阳"明显高于晚血并小三阳患者(P<0.01);晚血并HBV"小三阳"与晚血未感染HBV者无差异(P>0.05)。慢血并HBV"大三阳"与晚血并"大三阳"组比较,除HA外,其余3项指标前者明显低于后者(P<0.01);慢血并HBV"小三阳"与慢血并"大三阳"组比较,HA、IV.C前者明显低于后者(P<0.01),LN前者低于后者(P<0.05),PCIII两者无统计学意义(P>0.05);慢血未感染HBV组高于对照组(P<0.05);慢血并HBV"小三阳"组与慢血未感染HBV组比较,前者HA、PCIII明显高于后者(P<0.01),LN、IV.C无统计学意义(P>0.05);慢血未感染HBV组与控制组比较,除LN外,前者高于后者(P<0.05)。结论日本血吸虫病合并HBV感染后患者血清肝纤维化4项指标均增高,合并HBV"大三阳"者较合并HBV"小三阳"者增高更为明显。Objective To study four biomarkers level in serum for fiver fibrosis （HA.PC Ⅲ .LN.ⅣC） in both advanced and chronic schistosomiasis japonicum cases co-infected with HBV. Methods 125 advanced schistosomiasis, 145 chronic schistosomiasis and 50 healthy people were tested the levels in serum for liver fibrosis （HA.PC Ⅲ .LN.ⅣC） by radioimmunologT assay, and the biomarkers of Hepatitis B were detected by ELISA. Of the 125 advanced cases, 26 cases with HBsAg.HBeAg and HBcAb positive （group A）, 48 cases with HBsAg.HBeAb and HBcAb positive （group B） and 51 cases without HBV co-infection （group C ）. Of the 145 chronic cases, 25 cases with HBsAg.HBeAg and HBcAb positive （group D）, 35 cases with HBsAg. HBeAb and HBcAb positive （group E）, 85 cases without HBV co-infection（group F）. The 50 healthy people were selected as control group. Results The levels of four biomarkers in serum for liver fibrosis in group A ,B and C were higher than that in control group （P〈0.01）. Liver fibrosis markers in group A were apparendy higher than that in group B （P〈0.01）, and there was no statistic significance between group B and C （P〉 0,05 ）. The levels of biomarkers except HA in group D were significantly lower than that in group A （P〈 0.01 ）; The level of HA, IVC and LN in group E was significantly lower than that in group D （P〈0.05）, with no difference of PCⅢ level between two groups （P〉0.05）; the four biomarkers level in group F were higher than that in control group （P〈0.05）; The levels of HA.PC Ⅲ in group E were higher than that in group F （P〈0.01）, with no differences of LN.ⅣC level between two groups （P〉0.05）. The levels of HA. PCⅢ ,and ⅣC in group F were higher than that in control group（P〈0.05）. Conclusion The four biomarkers levels for liver fibrosis were remarkably higher for the schistosomiasis japonicum cases co-infected with HBV. EspeciaUy, the cases with HBsAg,HBeAg,HBcAb positive were significantly higher than these w

关 键 词：日本血吸虫病HBV 合并感染 肝纤维化 透明质酸 Ⅲ型前胶原 Ⅳ型胶原 层粘蛋白 

分 类 号：R512.62[医药卫生—内科学]