Effect of X-rays on Expression of Caspase-3 and p53 in EL-4 Cells and Its Biological Implications  

作　　者：GUI-ZHI JU Bo SHEN SHI-LONG SUN FENG-QIN YAN SUI-BO FU 

机构地区：[1]Department of Radiation Biology, School of Public Health, Jilin University, Changchun 130021, Jilin, China

出　　处：《Biomedical and Environmental Sciences》2007年第6期456-459,共4页生物医学与环境科学（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China (No. 30670630).

摘　　要：Objective To investigate the effect of X-rays on expression of caspase-3 and p53 protein in EL-4 cells and its implications in induction of apoptosis and polyploid cells. Methods Mouse lymphoma cell line （EL-4 cells） was used. Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression, apoptosis, cell cycle, and polyploid cells. Results The expression of caspase-3 protein increased significantly at 8 h and 12 h, compared with that of sham-irradiated control （P〈0.05, respectively） and the expression of p53 protein increased significantly at 2, 4, 8, 12, and 24 h, compared with that of sham-irradiated control （P〈0.05-P〈0.01） in EL-4 cells after 4.0 Gy X-irradiation. Apoptosis of EL-4 cells was increased significantly at 2, 4, 8, 12, 24, 48, and 72 h after 4.0Gy exposure, compared with that of sham-irradiated control （P〈0.05-P〈0.001）. G2 phase cells were increased significantly at 4, 8, 12, 24, 48, and 72 h （P〈0,05-P〈0.001）. However, no marked change in the number of 8 C polyploid cells was found from 2 to 48 h after 4.0 Gy exposure. Conclusion The expressions of caspase-3 and p53 protein in EL-4 cells are induced by X-rays, which might play an important role in the induction of apoptosis, and the molecular pathway for polyploid formation might be p53-independent.Objective To investigate the effect of X-rays on expression of caspase-3 and p53 protein in EL-4 cells and its implications in induction of apoptosis and polyploid cells. Methods Mouse lymphoma cell line （EL-4 cells） was used. Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression, apoptosis, cell cycle, and polyploid cells. Results The expression of caspase-3 protein increased significantly at 8 h and 12 h, compared with that of sham-irradiated control （P〈0.05, respectively） and the expression of p53 protein increased significantly at 2, 4, 8, 12, and 24 h, compared with that of sham-irradiated control （P〈0.05-P〈0.01） in EL-4 cells after 4.0 Gy X-irradiation. Apoptosis of EL-4 cells was increased significantly at 2, 4, 8, 12, 24, 48, and 72 h after 4.0Gy exposure, compared with that of sham-irradiated control （P〈0.05-P〈0.001）. G2 phase cells were increased significantly at 4, 8, 12, 24, 48, and 72 h （P〈0,05-P〈0.001）. However, no marked change in the number of 8 C polyploid cells was found from 2 to 48 h after 4.0 Gy exposure. Conclusion The expressions of caspase-3 and p53 protein in EL-4 cells are induced by X-rays, which might play an important role in the induction of apoptosis, and the molecular pathway for polyploid formation might be p53-independent.

关 键 词：X-rays CASPASE-3 P53 APOPTOSIS POLYPLOID 

分 类 号：Q2[生物学—细胞生物学]