内源性绵羊肺腺瘤病毒NM株pol基因的克隆与序列分析  

Cloning and sequence analysis of pol gene of the Inner Mongolia strain of endogenous jaagsiekte sheep retrovirus

作　　者：王宇[1] 刘淑英[1] 韩敏[1] 李建云[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《中国兽医杂志》2007年第12期8-10,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(30560108)

摘　　要：参照GenBank中内源性绵羊肺腺瘤病毒enJS56A1株pol基因序列设计了2对引物。应用PCR技术特异性地扩增出病毒的pol基因片段,将其克隆到PMD19-T载体后测序。应用计算机软件将测定序列与内源性南非代表毒株enJS56A1(AF153615)的pol基因序列比较,核苷酸同源性为99.3%,推导出的氨基酸同源性为95.0%。与外源性南非代表株JSRV-SA(M80216)的pol基因序列比较,核苷酸同源性为99.0%,氨基酸同源性为99.3%。这也是我国首次报道的内源性绵羊肺腺瘤病毒pol基因序列,为我国科研工作者进行更深入的研究奠定基础。According to the published pol gene sequence of enJS56A1 strain in GenBank, two pairs of primers were designed. A pol gene was amplified by PCR and then was cloned into PMD19-T vector and then sequenced. The acquired nucleotide sequences were analysed by computer softwares. As a result, The nucleotide and amino acid sequences of NM strain pol gene were compared with the counterpart sequences of South Africa enJS56A1 strain（AF153615） and South Africa JSRV-SA strain （M80216）. The nucleotide and amino acid homology of pol gene were 99.3%,95.0% and 98.8%,98.8%, respectively. This is the first nucleotide sequence of enJSRV reported in China. These results provide information for future developments on OPA.

关 键 词：内源性绵羊肺腺瘤病毒 pol基因 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]