产几丁质酶链霉菌SO1菌株的筛选及培养条件  

STUDY ON THE SCREENING AND CULTURE CONDITION OF STRPTOMYCES SP. SO1 STRAIN

作　　者：杨文博[1,2,3] 冯波[1,2,3] 佟树敏 张历[1,2,3] 

机构地区：[1]南开大学微生物学系 [2]天津市黄瓜研究所 [3]天津师范大学生物系

出　　处：《天津农业科学》1997年第3期1-4,共4页Tianjin Agricultural Sciences

摘　　要：从土样中分离获得的链霉菌ＳＯ１菌株在产酶培养基中，２８℃振荡培养７天，其胞外几丁质酶活力可达１８０９ｕ／ｍｌ。实验表明，不同碳、氮源及诱导物对ＳＯ１菌株产几丁质酶有不同的影响，以酵母粉对产酶的促进作用最为明显，比对照提高了７５．２％。部分脱乙酰几丁质、胶体几丁质及几丁质均可作为ＳＯ１菌株产几丁质酶的诱导物，其中以几丁质诱导产酶的效果最好。The chitinase activity of Strptomyces sp.SO1 which was obtained from soil could reach 1809u/ml after SO1 was cultivated in the shaker for 7 days at the temperature of 28℃.It was showed that different inducers,carbon and nitrogen sources had different impacts on SO1 strain.Yeast powder was the most distinctive factor,which enhanced the enzyme activity by 75.2% compared with the comparison.Partial deacetyl chitin,collodial chitin could be used as the inducers of SO1 strain for the production of chitinase.Among them,chitin was the best inducer.

关 键 词：链霉菌 几丁质酶 筛选 培养基 

分 类 号：Q939.13[生物学—微生物学] Q55