粪臭素对胰蛋白酶部分性质的影响  

The Partial Influence of Skatole on Trypsin

作　　者：梅宇[1] 赵巍[1] 刘芯韵[1] 李佳[1] 刘鑫[1] 刘克武[1] 

机构地区：[1]四川大学生命学院教育部生物资源与环境重点实验室,成都610064

出　　处：《天然产物研究与开发》2007年第6期1013-1015,共3页Natural Product Research and Development

摘　　要：研究发现粪臭素对胰蛋白酶活性具有抑制作用。采用紫吸收法察到粪臭素使胰蛋白酶紫外吸收峰增强,采用荧光光谱法发现粪臭素对胰蛋白酶有很强的荧光淬灭作用。根据Stern-Volmer方程测得胰蛋白酶的表观猝灭常数Kq为2.72×1012L/mol.s。表明粪臭素对胰蛋白酶的荧光猝灭很可能是静态猝灭。The prohibitive function of skatole over trypsin was studied by using ultraviolet spectroscopy and fluorescence spectrometry. It was found that skatole resulted in the increasing of UV absorption,and led to the quenching of intrinsic fluorescence of trypsin. The quenching constant Kq of pepsin obtained through stern-volmer plots is 2.72 × 10^12 L/mol · s. It was showed that the quenching of skatole on trypsin was probably a static quenching process.

关 键 词：粪臭素 胰蛋白酶 紫外光谱 荧光光谱 

分 类 号：Q556.9[生物学—生物化学]