粪臭素对胃蛋白酶部分理化性质的影响  

Spectroscopy Study on the Interaction between Skatole and Pepsin

作　　者：李政[1] 赵巍[1] 张蜀艳[1] 苏波[1] 冷小莲[1] 刘芯韵[1] 李佳[1] 刘鑫[1] 刘克武[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《天然产物研究与开发》2007年第4期599-602,共4页Natural Product Research and Development

摘　　要：采用紫外光谱法和荧光光谱法研究了粪臭素与胃蛋白酶的结合作用。观测到粪臭素使胃蛋白酶的紫外吸收峰增强,特征荧光峰淬灭。Stern-Volmer淬灭曲线显示,粪臭素对胃蛋白酶的荧光淬灭很可能是一个单一的静态淬灭过程。The binding interaction between skatole and pepsin was studied by ultraviolet absorption and fluorescence spectra. Skatole treatment led to the increasing of UV absorption and the quenching of intrinsic fluorescence of pepsin. The Stern-Volmer curve showed that the quenching of skatole to pepsin is probably a single static quenching process.

关 键 词：粪臭素 胃蛋白酶 紫外光谱 荧光光谱 

分 类 号：Q556.9[生物学—生物化学]