注射用红花提取物纯化方法优选  

Optimization of purification for injective extract from Carthamus tinctorius

作　　者：初阳[1] 宋洪涛[1] 李丹[2] 陈大为[2] 刘丹[2] 刘任[2] 

机构地区：[1]南京军区福州总医院药学科 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中草药》2007年第12期1804-1808,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30200363);辽宁省博士启动基金资助项目(2001102042)

摘　　要：目的优选注射用红花提取物的纯化方法。方法以红花黄色素(safflor yellow,SY)或羟基红花黄色素A(hydroxy safflor yellow A,HSYA)的转移率、质量分数,杂质的清除效果和复溶性作为考察指标,采用明胶沉淀法、澄清剂法、石硫醇法、碱性醇沉法和大孔吸附树脂法进行纯化,优选纯化条件,并对纯化结果进行比较。结果采用大孔吸附树脂纯化红花提取物,HSYA的转移率及纯度较高,杂质清除效果较好,复溶性较好。结论通过对不同纯化方法的比较,大孔吸附树脂法可提高红花提取物的纯化效果,优于其他纯化方法。Objective Optimizing the purification method for the injective extract from Carthamus tinctorius. Methods Taking the transfering rate, content, scavenging effect of impurities, and reconstitution of safflower yellow （SY） or hydroxysafflor yellow A （HSYA） as the indexes to optimize the effects of glutin precipitation, ZTC1＋1, SHILIU elcohol, basic precipitation, and macroreticular resin method for purification and to compare the results of the purification. Results When using macroreticular resin to purify the extract of C. tinctorius, the transfering rate and purity of HSYA were much higher. The scavenging effect of impurities and reconstitution were both good. Conclusions By comparison of different purification methods, it is found that the method using macroreticular resin could improve the purification and has a higher efficiency than others.

关 键 词：红花 红花黄色素 羟基红花黄色素A 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]