大豆种子脂肪氧化酶同工酶缺失体的超弱发光研究  

STUDY ON ULTRAWEAK BIOLUMINESCENCE OF SOYBEAN SEEDS LACKING ONE OR MORE LIPOXYGENASE ISOZYMES

作　　者：苏震[1,2,3] 徐文英 张仲纶[1,2,3] 马玉琴[1,2,3] 傅翠真 郑雁珍[1,2,3] 刘成祥 

机构地区：[1]中国科学院生物物理所 [2]北京植物细胞生物工程实验室 [3]中国农业科学院品种资源所

出　　处：《大豆科学》1997年第3期245-251,共7页Soybean Science

摘　　要：利用超弱发光方法对具有不同脂肪氧化酶同工酶缺失体的大豆种子进行整体水平上的测试，跟踪测量了在种子吸涨到萌动以及发芽的全过程中大豆种子超弱发光的变化，并且测定了它们的发光谱线。探索用发光强度鉴别是否有缺失Ｌｏｘ３异型材料的可能性和有效性；同时通过超弱发光发射光谱的差异来进一步研究不同大豆种子Ｌｏｘ缺失体的差异；试图开辟一条研究Ｌｏｘ生理意义的新途径。We used ultraweak bioluminescence (UWL) to analyze the soybean seeds lacking lipoxygenase (Lox) isozymes and detect their ultraweak ligth emission spectrum. The changes in ultraweak bioluminescence intensity and spectrum during germination of soybean seeds were studied UWL intensity and emission spectrum reflected the relationship between UWL and Lox. It is possible to detect soybean seed lacking L-3 by UWL intensity. Moreover, discussion is shown here on the physiological change during germination. This work may help us to research the physiology role of lipoxygenase.

关 键 词：超弱发光 大豆 种子 脂肪氧化酶 同工酶缺失体 

分 类 号：S565.103.2[农业科学—作物学]