氧化应激在实验性糖尿病肾病中的作用  

The Role of Oxidative Stress in the Experimental Diabetic Nephropathy

作　　者：王玲[1] 董德勇[1] 孙悦[2] 王永明[3] 

机构地区：[1]天津医科大学生理教研室,天津300070 [2]天津医科大学病理教研室,天津300070 [3]天津医科大学毒理教研室,天津300070

出　　处：《中国慢性病预防与控制》2007年第6期526-528,共3页Chinese Journal of Prevention and Control of Chronic Diseases

摘　　要：目的观察链脲佐菌素诱导的糖尿病大鼠不同时期肾组织中脂质过氧化物水平、抗氧化酶活性的动态改变,以探讨氧化应激在糖尿病肾病发生发展中的作用。方法测定糖尿病大鼠不同时期肾组织中丙二醛的含量和总超氧化物歧化酶的活性,同时观察糖尿病大鼠不同时期肾组织的组织形态学的改变。结果大鼠糖尿病1个月、3个月和6个月肾组织中丙二醛含量与对照组相比显著增高,分别为(2.16±0.57)和(1.18±0.18)nmol/mgprot,(2.03±0.39)和(1.63±0.14)nmol/mgprot,(2.09±0.26)和(1.59±0.14)nmol/mgprot,差别均有统计学意义(P<0.05或P<0.01)。糖尿病1个月肾组织总超氧化物歧化酶活性与对照组相比明显增高[(135.18±6.18)U/mgprot和(88.49±8.29)U/mgprot],差别有统计学意义(P<0.01),3个月时降至正常对照水平,6个月时显著降低(30.30±2.01)U/mgprot和(49.41±5.63)U/mgprot,差别有统计学意义(P<0.01)。组织形态学观察表明,糖尿病3个月时肾脏出现基底膜增厚、肾小球扩张等病理改变,随着病程的延长,病理改变越严重。结论糖尿病早期肾脏存在明显的氧化应激,氧化应激在糖尿病肾病的发生发展中起重要作用。Objective To explore the role of oxidative stress in the initiation and development of experimental diabetic nephropathy. Methods Levels of MDA and the total SOD activity were tested in the whole kidney at 1 month, 3 months and 6 months of experimental rat diabetes induced by streptozotocin. The pathological changes of diabetic rats were examined by light microscopy. Results The MDA level increased significantly in the diabetic group as compared to control group at all time intervals （2.16±0.57 vs 1.18±0.18, P〈0.01, 2.03±0.39 vs 1.63±0.14, P〈0.05, 2.09±0.26 vs 1.59±0.14, P〈0.01）. The total SOD activity increased significantly in the diabetic group as compared to control group at 1 month （135.18±6.18 vs 88.49±8.29,P〈0.01）,and dropped to baseline at 3 months, while decreased signif/cuntly at 6 months （30.30±2.01 vs 49.41±5.63, P〈0.01）. Inspection of the renal tissue by light microscopy demonstrated that the diabetic rats exhibited pathological changes at 3 months and the changes were more serious with the diabetic duration. Conclusion Oxidative stress was found to occur before the initiation of diabetic nephropathy and was maintained in the development of diabetic nephropathy. This implies that the oxidative stress plays an important role in the initiation and development of diabetic nephropathy.

关 键 词：糖尿病肾病 丙二醛 超氧化物歧化酶 

分 类 号：R587.1[医药卫生—内分泌]