高效液相色谱法测定注射用硫酸头孢噻利的含量  

Determination of Cefoselis Sulsate for lnjecton by HPLC

作　　者：卢协勤[1] 张根元[2] 

机构地区：[1]南京医科大学附属第二医院,江苏南京210011 [2]江苏省药物研究所,南京210009

出　　处：《抗感染药学》2007年第4期169-171,共3页Anti-infection Pharmacy

摘　　要：目的:建立高效液相色谱测定注射用硫酸头孢噻利含量的方法。方法:采用反相高效液相色谱法。固定相为十八烷基硅烷键合硅胶(150 mm×6 mm,Shim-Pack CLC-ODS),流动相为乙腈:pH 6.5磷酸盐缓冲液(取0.2 mol/L磷酸二氢钾50 mL与0.2 mol/L氢氧化钠溶液15.20 mL,混合,并加水至200 mL)(12:88),流量为1.0ml/min,检测波长为254nm。结果:硫酸头孢噻利的线性范围为2.084～18.756 lag/mL;相关系数为1.000。注射用硫酸头孢噻利平均含量为99.53%,相对平均偏差为0.39%。结论:该方法简便、准确、专属,可用于注射用硫酸头孢噻利的含量测定。Objective： To establish RP-HPLC method to determine the content of cefoselis sulfate for injecton. Methods： The RP-C8 Column（150 mm× 6 mm, Shim-Pack CLC-ODS）was used as the stationary phase with a mobile phase of acetonitrile ： pH 6.5 phosphate buffer （0.2 mol/L potassium dihydrogen phosphate 50 mL and 0.2 mol/L sodium hydroxide solution 15.20 mL water is added to 200 mL）（12：88）.The flow rate was 1.0 ml/min and the detection wavelength was 254 nm. Results：The linear range was 2.084-18.756 μg/mL; correlation coefficients were 1.000. The average content（n = 6） of cefoselis sulfate was 99.53% and RSD was 0.39%. Conclusion： The mothod is sample, accurate and specific. It can be used for the determination of the cefoselis sulfate for injecton.

关 键 词：高效液相色谱法 注射用硫酸头孢噻利 含量 

分 类 号：R978.11[医药卫生—药品]