血管紧张素Ⅱ1型受体基因多态性与高龄原发性高血压的相关性  

Relationship between polymorphism of AT_1 receptor gene and the essential hypertension in very old patients

作　　者：李金珉[1] 路方红[1] 刘振东[1] 杨建民[1] 周晓红[1] 

机构地区：[1]山东省医学科学院心脑血管病防治研究中心山东省现代医用药物与技术实验室,济南250062

出　　处：《中华老年心脑血管病杂志》2007年第12期800-803,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的研究血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与汉族高龄原发性高血压的相关关系。方法采用PCR-RFLP方法,对山东省章丘地区高龄原发性高血压患者104例(高龄高血压组)和体检正常老年人98例(正常对照组)的外周血白细胞DNA,进行AT1R基因A1166C多态性检测,观察AA、AC和CC不同基因型以及该点A、C不同等位基因频率在两组中的分布。结果AA、AC和CC基因型在高龄高血压组的分布为83(79.81%)、20(19.23%)和1(0.96%),正常对照组为82(83.67%)、16(16.33%)和0,两组比较差异无显著性意义(χ2=0.50,P>0.05);A1166与C1166等位基因频率在高龄高血压组中分别为186(89.42%)和22(10.58%),正常对照组为180(91.84%)和16(8.16%),两组比较差异无显著性意义(χ2=1.38,P>0.05)。结论AT1R基因A1166C分子变异与山东省章丘地区汉族高龄原发性高血压无相关性。Objective To investigate the relationship between the A1166C allele polymorphism of angiotensin Ⅱ type 1 receptor（AT1R）gene and the essential hypertension in very old patients. Methods Polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） methods were used to detect the polymorphism of AT, R gene in 104 very old patients with hypertension and 98 normotensive controls. Results The genotype frequencies of A1166C were AA83 （79.81%）,AC20（19. 23%） and CCI（0.96%） in very old patients group and the corresponding frequencies were 82（83.67%）, 16（16.33%） and 0 in the control group respectively. The A1166 and C1166 allele frequencies were 186（89.42%） and 22（10.58%） respectively in very old patients group and 180（91.84%） and 16（8.16%） in normotensive group. The A1166C genotype distribution and the allele frequencies of A1166 and C1166 had no statistically significant difference in very old patients group compared with normotensive group（ P 〉0.05）. Conclusion There was no significant association between the Al166C polymorphism of AT1 R gene and the essential hypertension in very old patients in Zhangqiu of Shandong province.

关 键 词：高血压 受体 血管紧张素 1型 基因频率 危险因素 

分 类 号：R544.1[医药卫生—心血管疾病]