自动化微量板法检测红细胞不规则抗体的研究  

Study of a New Automation Microplate Method for Screening Red Cell Irregular Antibody

作　　者：孙爱农 黄丽雅[2] 许惠根 冯志广 廖艳婷 何锐洪 

机构地区：[1]中山市红十字中心血站,广东中山528403 [2]中山市人民医院,广东中山528400 [3]中山火炬开发区医院,广东中山528437

出　　处：《现代检验医学杂志》2007年第6期9-12,共4页Journal of Modern Laboratory Medicine

基　　金：中山市科技计划基金资助项目(No.2005A138)

摘　　要：目的利用自动化设备寻找一种简便快速的献血者红细胞不规则抗体(RBC-IAb)筛选方法。方法采用微量板聚凝胺三步法试验(TSPT)、菠萝酶法、抗人球蛋白法(AGT)及经典试管TSPT,平行检测一批Rh等抗体效价,选出最佳的筛查方法,并观察抗凝剂、溶血、脂血对实验的影响。结果在上述四种方法中,以微量板TSPT作为筛检最佳,对14 210名献血者进行了RBC-IAb筛查,发现40例阳性,最多为抗-Mur 15例。结论微量板TSPT筛查献血者RBC-IAb,操作简便迅速,敏感性高,可在自动化加样仪中使用,并能与酶标仪和计算机相结合,尤其适合大批标本筛查。Objective The usage automates the equipments ,look for one kind of simple and fast and the sensitive red blood cell irregular antibody （RBC-IAb） screening testing method. Methods Used microplate three-stage polybrene test （TSPT）,bromelin technology,the antl-globulin test （AGT） and the traditional test tube （TSPT）,paralleled determines the titre of Rh ,MNSs ,Duffy ,Kidd blood system irregular antibody ,observed the sensitivity and specificity of assay ,selected the best screens test method ,and observed influence of the anticoagulant and hemolysis ,the fat the blood for the experiment. Results In above four kinds of methods,microplate TSPT screens test was best,carried on the RBC-IAb screens test to 14210 donor who donated blood for volunteer,discovered 40 positive,most was anti-Mur（15 samples）. Conclusion The microplate TSPT of this text screening red cell irregular antibody, operate simple quick,the sensitivity is high,can in automate add the kind instrument usage,and can combine together with the ELISA colorimeter instrument （fully automatic ,microprocessor controlled reader）and computer ,is particularly suitable for the large quantity specimen screening test.

关 键 词：红细胞不规则抗体(RBC—IAb) 筛选试验 微量板 聚凝胺三步法试验(TSPT) 自动化 

分 类 号：R457.12[医药卫生—治疗学] R446.62[医药卫生—临床医学]