兔动脉粥样硬化进程中红细胞Na^+-K^+-ATPase和全血粘弹性参数的变化及其可能的预示作用  

Changes of erythrocyte Na+K+ATPase and whole blood viscoelastic parameters in the course of experimental atherosclerosis in rabbits and their possible predicting role

作　　者：王红勇[1,2] 何作云[1,2] 丁秋华[1,2] 黄永萍[1,2] 

机构地区：[1]第三军医大学附属大坪医院心血管内科 [2]第三军医大学附属新桥医院

出　　处：《第三军医大学学报》1997年第3期213-216,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金

摘　　要：目的：了解血脂、Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ及全血粘弹性参数（弹性模量及动粘度）在兔动脉粥样硬化进程中的预示作用。方法：健康家兔８０只随机分为实验组（ＡＧ）６０只及对照组（ＣＧ）２０只，各组又分为４个亚组，即ＡＧ１～ＡＧ４和ＣＧ１～ＣＧ４，ＡＧ给予胆固醇粉１ｇｄ－１只－１，在喂养的第２、４、８和１２周分别观察ＡＧ１～ＣＧ１、ＡＧ２～ＣＧ２、ＡＧ３～ＣＧ３、ＡＧ４～ＣＧ４主动脉粥样硬化（ＡＳ）病变和上述指标的变化。结果：在兔ＡＳ阶段性进程中，血脂（Ｔｃｈ、ＴＧ）在ＡＳ早期明显增高，其后有所降低；红细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ活性自ＡＳ早期起进行性降低，且在ＡＳ早、晚期与Ｔｃｈ有密切负相关关系（ｒ＝－０．６７、－０．５１，Ｐ＜０．０１、０．０５）；全血粘弹性参数中，在早期仅当角频率（ω）为０．１９０Ｈｚ时弹性模量呈单一性升高，而中、晚期弹性模量及动粘度普遍升高，二者之间有正相关关系（ｒ＝０．５１～０．８５，Ｐ＜０．０５～０．００１）。结论：上述指标在ＡＳ进程中有一定的阶段性预示作用。Objective: The role of blood lipids, Na+K+ATPase activity, whole blood dynamic viscosity (η′) and dynamic modulas (G′) in predicting different stages of experimental atherosclerosis (AS) were studied. Methods: 80 rabbits were randomly divided into experimental group (n=60) and control group (n=20). Each of the two groups was also equally divided into for subgroups: four experimental subgroups and four control ones. Each rabbit of the experimental group was fed with cholesterol powder (1g/d). At the 2nd, 4th, 8th and 12th week, blood lipid concentration, erythrocyte Na+K+ATPase activity and whole blood viscoelasticity were tested and the pathologic alteration of AS in the aorta were also observed. Results: Serum triglyceride (TG) was increased only in the early stage of AS and serum total cholesterol (Tch) was significantly higher in the early, middle and late stage of AS than in controle. Erythrocyte Na+K+ATPase activity was progressively decreased and negatively correlated with Tch in the early and late stage of AS(r=-0.67、-0.51, P<0.01,0.05) G′ was significantly increased only when shear rate was 0.190 Hz in early AS, but G′ and η′ were progressively elevated in the middle and late stage of AS. Conclusion: It is possible that blood lipids, Na+K+ATPase activity and whole blood viscoelasticity can predict the various stages of experimental atherosclerosis in rabbits.

关 键 词：动脉粥样硬化 血脂 全血粘弹性 钠 钾 ATP酶 

分 类 号：R543.502[医药卫生—心血管疾病]