体外培养人外周血白细胞中阿德福韦酯诱生干扰素的作用研究  

Effects of adefovir dipivoxil on the secretion of interferon-γ(IFN-γ) in culturing white blood cells

作　　者：江军[1] 缪晓辉[2] 蔡雄[2] 王雯婧[1] 

机构地区：[1]解放军第113医院感染科,浙江宁波315040 [2]长征医院感染科,上海200003

出　　处：《临床肝胆病杂志》2007年第6期430-432,共3页Journal of Clinical Hepatology

摘　　要：目的观察阿德福韦酯对慢性乙型肝炎(CHB)患者和健康人体外培养的外周血白细胞(WBC)作用后,培养上清中干扰素活性的动态变化,探讨阿德福韦酯是否能诱导WBC产生干扰素。方法抽取20名CHB患者和20名健康人的外周血,将白细胞放入有不同浓度阿德福韦酯(5μg/ml、7.5μg/ml、10μg/ml及无药对照组)的培养液中,分别于24、48及72小时三个时间点利用微量细胞病变抑制法(CPE)测定培养液中IFN的效价。结果健康人组中,24小时点三组均高于无药对照组(P<0.001),其中5μg/ml组72小时点与24小时点比有明显升高(P=0.004);CHB患者组中,各时间点不同浓度阿德福韦酯组与对照组无差异。结论阿德福韦酯有一定诱生正常人WBC分泌干扰素的作用,而对CHB患者的WBC则此作用不明显。Objective To observe whether adefovir dipivoxil has antiviral immunological effect in vitro culturing white blood cells of patients with chronic hepatitis B（CHB） and normal people by determining the IFN activity in culture medium. Methods The blood specimen were gathered from twenty CHB patients and twenty health people, then WBC were cultured in medium containing adefovir dipivoxil （5ug/ml, 7.5ug/ml and 10ug/ml） and control group with no adefovir dipivoxil. At different time （24h,48h,72h） after agent added, culture medium were harvested. Meanwhile, IFN activity of each sample was determined by cytopathic effect inhibition assay. Results At different time after drugs were added, in health people, the titers of IFN activity in drug groups（5ug/ml, 7.5ug/ml and 10ug/ml） improved significantly at 24h （P 〈0. 001 ） , in 5ug/ml group it was significantly higher at 72h hours than that at 24h（ P =0. 004） ;there were no significant difference between drug groups and control groups in CHB patients. Conclusion Adefovir dipivoxil can induce WBC of healthy people to secrete interferon to certain extent, but this function isnt significant in WBC of patients with CHB.

关 键 词：福韦酯 病毒性肝炎 乙型 慢性 外周血白细胞 干扰素 

分 类 号：R96[医药卫生—药理学]