实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能  

Recent thymic output function in patients with chronic hepatitis C by real time fluorescence quantitative PCR analysis

作　　者：郭海燕[1] 陈明[1] 汪莉萍[1] 葛国洪[1] 张言超[1] 

机构地区：[1]徐州医学院附属医院感染病科,江苏徐州221002

出　　处：《临床肝胆病杂志》2007年第6期432-433,共2页Journal of Clinical Hepatology

基　　金：江苏省科委社会发展计划基金(BS2004014)

摘　　要：目的观察慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)的含量,从而推测CHC患者的胸腺近期输出功能,并且比较TRECs含量与HCV载量的关系。方法利用实时荧光定量PCR方法检测22例CHC患者及16例正常人PBMCs中TRECs含量,用RT-PCR方法结合荧光探针的检测技术检测CHC组外周血血清中的HCVRNA。结果CHC患者中TRECs含量为(5.52±1.87)copies/103PBMCs,低于正常人TRECs水平(7.19±2.42)copies/103PBMCs,P<0.05;TRECs含量与HCV载量直线相关分析r=-0.427,P<0.05。结论CHC患者TRECs水平低于正常人,显示其胸腺近期输出功能明显降低,且与HCV载量呈负相关性。Objective To analyze the content of T - cell receptor excision circles （TRECs） within peripheral blood mononuclear cells of chronic hepatitis C （CHC） patients to value the thymic output function of CHC and investigate the relation of thymic output function and HCV RNA in chronic hepatitis C （CHC） patients. Methods Quantitative detection of TREcs in DNA of peripheral blood mononuclear cells（PBMCs） from 22cases with CHC, 16 normal individuals was performed by real - time fluorescence quantitative PCR, and detection of HCV RNA of peripheral blood serum in CHC by RT - PCR with fluorescent probe technology. Results The mean of TRECs in patients with CHC （5. 52 ± 1.87 copies/10^3 PBMCs） was lower than that in normal individuals （7. 19±2.42 copies/10^3 PBMCs, P 〈0.05） ; The value of TRECs in patients with CHC was inversely correlated with HCV RNA（γ = -0. 427,P 〈 0.05 ）. Conclusion TRECs levels in CHC are lower in the healthy and inversely correlated with HCV RNA.

关 键 词：慢性丙型肝炎 TRECS 胸腺近期输出功能 实时荧光定量PCR 

分 类 号：R512.6[医药卫生—内科学]