应用免疫磁珠法分离脐血CD_(34)^+造血细胞  

IMMUNOMAGNETIC POSITIVE SELECTION OF CD_(34)^+ PROGENITOR CELLS FROM HUMAN CORD BLOOD

作　　者：董文川[1] 喻新建[1] 童春容[1] 高晖[1] 陈珊珊[1] 陆道培[1] 

机构地区：[1]北京医科大学人民医院血液病研究所,100044

出　　处：《北京医科大学学报》1997年第3期275-277,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：分离脐血CD细胞，以了解其造血增殖活性。方法：应用免疫磁珠法（Isolex50系统）分离脐血CD细胞，并对其实验方法进行了改进。结果：常规方法CD纯度较低，经过改进，脐血CD细胞由分离前的（1．39±0．76）％增加至（59．46±17．35）％，CD细胞富集倍数为（62．31±55．19）倍。分离后组分CFU－GM、BFU－E、CFU－Mix较分离前分别增加了（41．05±15．77）、（13．14±8.66）及（34．80±19．58）倍，而阴性组分所形成的集落显著减少。结论：免疫磁珠法可以有效地分离CD细胞，造血祖细胞集落大多来源于CD细胞。objective: The experiments aimed at the separation of CD cells from human cord blood and evaluation of its clonogeneic ability. Methods: Immunomagnetic bead separation (Isolex50 systere) was used to enrich CD cells. Results: By improved technique, the purity of CD cells separated from the mononuclear cells of the cord blood was (59.46±17. 35) % [starting population (1. 39±0. 76) %].With the separated CD cell fraction from cord blood, CFU-GM, BFU-E and CFU-Mix were enriched to (41.05±15. 77) fold, (13. 14±8. 66) fold, and (34. 80±19. 58) fold, respectively; while CD fraction developed only a few colonies. Conclusion: Immunomagnetic bead separation was effective in separating CD cells from cord blood, and CD cells enriched most of the clonogeneic cells.

关 键 词：造血干细胞 脐带血 免疫磁珠法 CD34 分离 

分 类 号：R331.2[医药卫生—人体生理学]