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机构地区:[1]中国科学院西北高原生物研究所
出 处:《西北植物学报》2007年第12期2491-2495,共5页Acta Botanica Boreali-Occidentalia Sinica
基 金:中国科学院知识创新工程重要方向性项目(KSCX2-SW-304);中科院中组部西部之光联合学者项目资助
摘 要:以高原448小麦品种为材料,分别测定了4个处理(Hoagland营养液、Hoagland营养液+150 mmol/LNaCl、Hoagland营养液+150 mmol/L NaCl+10μmol/L H2O2和Hoagland营养液+10μmol/L H2O2)的小麦幼苗在第2、4、6、8天叶片叶绿素、丙二醛、可溶性糖和还原性谷胱甘肽含量.结果显示:外源H2O2提高了NaCl胁迫下4个时段小麦幼苗的叶绿素含量(8.27%、32.57%、10.19%、4.86%)及还原性谷胱甘肽含量(3.09%、23.97%、5.85%、2.11%),显著提高了可溶性糖含量(14.58%、8.43%、16.68%、5.8%,P<0.05),而显著降低了其丙二醛含量(17.53%、14.04%、4.75%、8.47%,P<0.05).外源H2O2(10μmol/L)使NaCl胁迫下叶绿素含量和还原性谷胱甘肽含量峰值提前,同时推迟了丙二醛峰值出现的时间.研究表明,外源H2O2通过提高叶片叶绿素、可溶性糖和还原性谷胱甘肽含量以有效地增强小麦幼苗的耐盐性.The chlorophyll content,MDA content, soluble sugar content and GSH content were determined at the 2^nd ,4^th, 6^th and 8^th day, respectively, after treatments of Hoagland solution, Hoagland solution+ 150 mmol/L NaC1, Hoagland solution + 150 mmol/L NaCl + 10 μmol/L H2O2 and Hoagland solution + 10 μmol/L H2O2. The addition of exogenous H2O2 (10 μmol/L) increased the wheat seedling chlorophyll content (by 8. 27%,32.57%,10. 19% and 4. 86%) and GSH contents (by 3. 09%,23. 97%,5. 85% and 2. 11%),dramatically increased soluble sugar content (by 14.58%,8. 43%,16.68% and 5.8%,P〈0.05) and dramatically decreased MDA content (by 17.53%,14.04%,4.75% and 8.47%,P〈0.05) compared to the salt stressed (Hoagland solution+NaCl) group on the 2^nd, 4^th, 6^th and 8^th day, respectively, after treat- ments. Exogenous H2O2 (10 μmol/L) accelerated the maximum accumulation chlorophyll and GSH contents in NaCl stressed condition while decelerated the maximum accumulation content of MDA. Exogenous H2O2 enhanced wheat seedling salinity tolerance.
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