数目可变串联重复序列用于江苏省168株结核分枝杆菌菌株的基因分型研究  

Genotyping study of 168 strains of Mycobacterium tuberculosis isolated from Jiangsu province in VNTR

作　　者：许卫国[1] 虞浩[1] 杨丹丹[1] 吕华坤[1] 周扬[1] 

机构地区：[1]江苏省疾病预防控制中心慢传科,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2007年第12期1509-1512,1516,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30471526);江苏省卫生厅科研基金资助项目(H200335)。

摘　　要：目的:初步了解江苏省结核分枝杆菌临床分离菌株的数目可变串联重复序列(Variable number of tandem repeats,VNTR)的基因型及VNTR基因分布特征。方法:在苏南、苏中、苏北三个地区13个结核病定点诊疗单位收集临床分离菌株。选取结核分枝杆菌基因组中11个具有明显多态性特征的VNTR位点,通过PCR扩增,2%琼脂糖凝胶电泳和Bio Numerics(Version 3.0)软件进行结核菌株VNTR的多态性和聚类分析。结果:共收集到168株结核分枝杆菌。不同的菌株在同一位点上具有多态性。共分为10个基因型(Ⅰ,Ⅱ～Ⅹ型),其中以Ⅷ型为主要基因型,占75.0%、其次为Ⅱ型5.4%、Ⅳ型4.2%、Ⅶ型9.5%。不同地区之间,苏南、苏中、苏北三个地区也都以Ⅷ型为主要基因型,分别占各地区的83.5%,57.1%,76.6%;但是苏南、苏中、苏北三地的Ⅱ、Ⅲ、Ⅳ、Ⅶ型菌株分布在各地菌株中的比例有差异显著性((2=54.710,P<0.0001),Ⅱ型以苏中为主(11.9%)、Ⅲ型以苏南为主(3.8%)、Ⅳ型以苏南、苏北为主(5.1%与6.4%)、Ⅶ型以苏中为主(31.0%)。结论:江苏省的结核分枝杆菌具有明显的多态性,以Ⅷ型为主要流行型,不同地区间同样以Ⅷ型为主要流行型,但菌型分布存在一定的差异。Objective：To investigate the distribution of the genotyping and the characteristics of VNTRs in clinical Mycobaterium tuberculosis （MTB） strains isolated from Jiangsu province with multi-loci of variable number tandem repeats. Methods： 168 strains of Mycobacterium tuberculosis were selected from three parts of Jiangsu province. Eleven loci of VNTR from the genome of Mycobacterium tuberculosis were selectd and analyzed for the polymorphism in distribution by means of PCR, agarose electrophoresis and the BioNumerics （Version 3.0）software. Results：It was found that distribution of VNTR from 168 strains of Mycobacterium tuberculosis isolated showed definite polymorphism,and 10 different genotypes could be classified in which most of strains belonged to one genotype（75.1%）,and the others were scattered,such as Ⅱ （5.4%）, Ⅳ （4.2%）, Ⅶ （9.5%）. We also found that there was visiblely different among three parts of Jiangsu province. Conclusion：It is concluded that the VNTR loci in the genome of Mycobacterium tuberculosis isolated in Jiangsu showed marked polyporphism, most of the 168 strains belonged to the one genotype （Ⅷ）. The distribution of main prevalent strains among three parts were the same（Ⅷ）, but other genotypes distribution of strains were different.

关 键 词：结核分枝杆菌 多位点数目可变串联重复序列分析 基因分型 

分 类 号：R181.81[医药卫生—流行病学]