利用鱼粉生产中的废水培养苏云金芽胞杆菌的可行性研究  

Feasibility of bacterium Bacillus thurngiensis culture by effluents from a fishmeal factory

作　　者：张东升[1] 曹文伟[2] 毛连菊[1] 朱莹[1] 

机构地区：[1]大连水产学院农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁大连116023 [2]辽宁师范大学生物系,辽宁大连116026

出　　处：《大连水产学院学报》2007年第6期442-445,共4页Journal of Dalian Fisheries University

基　　金：辽宁省科学技术基金项目(9910600102)

摘　　要：用正交试验L9(43)设计方法,对用鱼粉生产中的废水培养苏云金芽胞杆菌Bacillus thuringiensis进行了可行性试验和初步优化试验。结果表明:用鱼粉生产中的废水培养苏云金芽胞杆菌是可行的,在(28±1)℃条件下,在含有50%的鱼粉废水中添加10 g/L的水淀粉、1 g/L CaCO3、0.25 g/L MgSO4、0.25 g/LKH2PO4作为培养基培养苏云金芽胞杆菌,其数量可达2.72×109cell/mL,且芽胞脱落时间一致,脱落率为99%。Feasibility and optimization of bacterium Bacillus thurngiensis culture by effluents from a fishmeal factory were conducted by a method of orthogonal test L9 （4^3）. The results showed that the bacterium was cultured by a medium from the fishmeal factory effluent. The B. thurngiensis density of 2.72 × 10^9 cell/mL and the mature spore ratio of 99% were observed in the effluent medium containing 50% fishmeal factory effluent, 10 g/L solubility amylum, 1 g/L calcium carbonate, 0.25 g/L magnesium sulphate, 0.25 g/L, and monopotassium phosphate at temperature of （ 28 - 1 ）℃.

关 键 词：鱼粉废水 苏云金芽胞杆菌 正交试验 可行性 

分 类 号：S917.5[农业科学—水产科学]