光谱法研究氟虫腈与牛血清白蛋白的相互作用  

Study on the Interaction between Fipronil and Bovine Serum Albumin by Spectroscopy

作　　者：童金强[1] 张华新[2] 梅平[1] 

机构地区：[1]长江大学化学与环境工程学院,湖北荆州434023 [2]荆楚理工学院化工学院,湖北荆门448000

出　　处：《化学与生物工程》2007年第12期61-63,共3页Chemistry & Bioengineering

摘　　要：在模拟人体生理条件下,采用荧光光谱法和紫外可见吸收光谱法研究了氟虫腈与牛血清白蛋白的相互作用。结果表明,氟虫腈对牛血清白蛋白的内源荧光有一定的猝灭作用,由Stern-Volmer方程和Lineweaver-Burk方程分析处理实验数据,得到了结合作用的平衡常数、结合反应的热力学参数、结合位置、结合作用力类型等。The interaction between fipronil and bovine serum albumin（BSA） was studied using fluorescence and UV-visible spectroscopy under imitating human body physiology environment. The results showed that fipronil had a powerful ability to quench the BSA fluorescence via a non-radioactive energy transfer mechanism. By analyzing the spectral data with Stern-Volmer equation and Lineweaver-Burk equation, the static fluorescence quenching binding constant, the binding locality, the thermodynamic parameters, and the type of binding power were obtained.

关 键 词：氟虫腈 牛血清白蛋白 荧光光谱法 热力学参数 

分 类 号：Q512.1[生物学—生物化学] O657.3[理学—分析化学]