环氧化合物制备丝素凝胶材料的细胞毒性研究  

Research on Cytotoxicity of Silk Fibroin Gel Materials Prepared with Polyepoxy Compound

作　　者：田莉[1] 陈宇[2] 闵思佳[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江省中医药研究院,杭州310007

出　　处：《生物医学工程学杂志》2007年第6期1309-1313,共5页Journal of Biomedical Engineering

基　　金：浙江省科技计划资助项目(2007C21128)

摘　　要：利用环氧化合物与丝素蛋白在冰点以下或较高温度下作用可以获得两种不同结构和性能的凝胶材料。为研究两种材料在生物医用材料方面应用的可行性,本实验测定了两种凝胶材料的浸提液对小鼠成纤维细胞(L-929)细胞增殖率的影响,初步探索了两种凝胶材料的细胞毒性,发现两种凝胶材料均呈现出较高的细胞相对增殖率,细胞毒性均为1级,都在可以医用的合格范围内;另外实验还对环氧化合物和戊二醛两种交联剂的细胞毒性进行了对比,发现环氧化合物的细胞毒性明显低于戊二醛,认为环氧化合物可以作为丝素蛋白材料改性的更为安全的交联剂。Two kinds of gel materials were prepared from silk fibroin with polyepoxy compound at subfreezing temperature and higher temperature. In order to evaluate the feasibility of their application in biomaterials, we tested the eytotoxieity of silk fibroin gels by detecting the effect of the extracted liquid on the cell relative proliferation rate of L-929 mouse fibroblasts. The results indicated that both of the gel materials displayed high relative proliferation rate and grade 1 eytotoxieity, being in the allowed range of medical application. The eytotoxicity tests on polyepoxy compound and glutaraldehyde were conducted too, and the cytotoxicity of polyepoxy compound was obviously lower than that of glutaraldehyde. Polyepoxy compound can be used as a more safe cross-llnk reagent for silk fibroin modification.

关 键 词：丝素蛋白 化学交联 细胞毒性 

分 类 号：R318.08[医药卫生—生物医学工程]