犬骨髓间充质干细胞定向分化为内皮细胞的研究  

Investigation of Canine Mesenchymal Stem Cells Differentiation to Vascular Endothelial Cell in Vitro

作　　者：李清刚[1] 徐秀红[2] 王质刚[1] 刘文虎[1] 李哲先[2] 

机构地区：[1]北京友谊医院肾内科,北京100050 [2]北京友谊医院泌尿外科,北京100050

出　　处：《生物医学工程学杂志》2007年第6期1348-1351,共4页Journal of Biomedical Engineering

基　　金：北京市科技新星计划基金资助(H013610280112)

摘　　要：应用密度梯度离心法分离狗骨髓间充质干细胞,建立诱导分化内皮细胞的方法及条件。密度梯度离心法分离狗骨髓间充质干细胞后,在体外经血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)、内皮细胞生长因子(Endothelial growth factor,EGF)等诱导后,分化形成内皮样细胞。结果表明:光镜下细胞单层融合生长,呈铺路石样形态,单个核位于中央。电镜下可见到Weibel-Palade小体,在细胞胞浆vWF染色阳性。骨髓间质干细胞,在体外可诱导分化成内皮细胞。To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells （MSCs） were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor （VEGF）, endothelial growth factor （EGF） and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope, vWF,a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.

关 键 词：骨髓间充质干细胞 内皮细胞 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]