HO-1在CCK-8减轻脂多糖所致的急性肺损伤中的作用  

Role of heme oxygenase in cholecystokinin octopeptide ameliorating acute lung injury induced by lipopolysaccharide

作　　者：黄新莉[1] 韦鹏[1] 周晓红[1] 凌亦凌[1] 

机构地区：[1]河北医科大学病理生理教研室,河北石家庄050017

出　　处：《中国病理生理杂志》2007年第12期2385-2389,共5页Chinese Journal of Pathophysiology

基　　金：河北省科技厅资助项目(No052761344);河北省卫生厅资助项目(No05114)

摘　　要：目的:探讨血红素氧合酶(HO)-1在八肽胆囊收缩素(CCK-8)减轻脂多糖(LPS)所致急性肺损伤(ALI)中的作用。方法:将大鼠随机分为5组:正常对照组、LPS组、CCK-8+LPS组、LPS+Hm(氯血红素,CO供体)组、LPS+ZnPP(锌原卟啉,HO-1特异性阻断剂)组。各组给药后2h、6h、12h行支气管肺泡灌洗、检测支气管肺泡灌洗液(BALF)中中性粒细胞(PMN)数目;进行肺组织的形态学观察;测定肺组织中丙二醛(MDA)含量和HO-1蛋白活性;应用RT-PCR和Western blotting技术检测给药后6h肺组织中HO-1 mRNA和蛋白的表达情况。结果:LPS组肺组织出现损伤性变化,同时BALF中PMN数目、肺组织中MDA含量、HO-1蛋白活性、HO-1 RNA和蛋白的表达均高于相应对照组(均P<0.05);CCK-8+LPS和LPS+Hm组肺组织损伤程度、BALF中PMN数目和肺组织中MDA含量低于相应LPS组,而肺组织中HO-1蛋白活性、HO-1 mRNA和蛋白的表达均高于相应LPS组(均P<0.05);LPS+ZnPP组肺组织损伤程度、BALF中PMN数目和肺组织中MDA含量分别高于相应LPS组,而肺组织中HO-1蛋白活性、HO-1 mRNA和蛋白的表达分别低于相应LPS组(均P<0.05)。结论:CCK-8可部分通过HO-1介导的抗氧化、抑制PMN聚集等效应来发挥减轻LPS所致的肺损伤作用。AIM： To study the role of heme oxygenase （HO） - 1 in the mechanism of cholecystokinin - octapeptide （CCK -8 ） for attenuation of acute lung injury （ALI） induced by lipopolysaccharide （LPS）. METHODS： Adult male rats were randomly divided into five groups ： control group, LPS group, CCK - 8 ＋ LPS group, LPS ＋ Hm （ hemin, HO - 1 donor） group and LPS ＋ ZnPP （ zinc protoporphyrin, specific inhibitor of HO - 1 ） group. PMN number in bronchoalveolar lavage fluid （ BALF）, the structure of the lung, MDA content, HO - 1 activity, the expressions of HO - 1 mRNA and protein in the lung were detected respectively. RESULTS： The lung injury in LPS group was observed, at the same time the numbers of PMN, the content of MDA, the activity and the expression of HO - 1 were all higher than those in control group （ all P 〈0. 05）. The degree of lung injury, PMN numbers and MDA content were lower, while the activity and the expression of HO - 1 in CCK - 8 ＋ LPS and LPS ＋ Hm group were higher than those in LPS group （ all P 〈 0. 05 ）. However, the degree of lung injury, PMN numbers and MDA content were higher, the activity and the expression of HO 1 were lower in LPS ＋ ZnPP than those in LPS group respectively （ all P 〈 0. 05 ）. CONCLUSION ： CCK - 8 attenuates the LPS - induced ALI by means of anti - oxidation and inhibits PMN aggregation, which are both mediated by HO - 1 partly.

关 键 词：脂多糖类 急性肺损伤 胆囊收缩素 血红素氧化酶-1 

分 类 号：R543.2[医药卫生—心血管疾病]