姬菇原生质体制备与再生条件初探  

Protoplast Preparation and Regeneration of Pleurotus cornucopiae

作　　者：杨凤苓[1] 贾乐[1] 任轩[1] 

机构地区：[1]山东农业大学生命科学学院,山东泰安271018

出　　处：《生物技术》2007年第6期37-40,共4页Biotechnology

基　　金：山东省自然基金项目资助(Y2006D08)

摘　　要：目的:探索姬菇(Pleurotus cornucopiae)菌丝原生质体制备和再生条件。方法:以培养5d的幼嫩菌丝体为材料,在1.5%溶壁酶+0.5%纤维素酶混合酶作用下,以0.6mol/L甘露醇作渗透压稳定剂于pH5.5,30℃酶解2.5h,然后用血球计数板计数,计算原生质体产量。结果:在上述条件下,原生质体产量达到3.12×107个/mL。原生质体适宜的再生培养基为:马铃薯200g,蔗糖20g,酵母膏2g,KH2PO43.0g,MgSO4.7H2O1.5g,维生素B10.1g,0.6mol/L甘露醇。采用上述培养基,原生质体再生率达到0.78%。为姬菇原生质体诱变及融合育种奠定了基础。Objective： The optimum conditions of isolation and regeneration of protoplasts from the mycelia of Pleurotus cornucopiae were studied. Methods：Maximum yield of protoplasts could be obtained with 5d- mycelia by using enzyme system composed of 1.5% lywallzyme and 0.5% cellulase with 0.6mol/L mannitol being used as osmotic stabilizer at 30℃ for 2.5h. Results：The highest protoplast yield and regeneration rate of Pleurotus cornucopiae was 3.12×10^7 and 0.78% on RM2. The compotent of this medium was potato 200g, sucrose 20g, yeast extracts 2g, KH2PO4 0.3g, MgSO4 ·7H2O 1.5g, vitamin B1 0. 1g, 0.6mol/L mannitol as osmotic stabilizer. These results would provide important parameters for the strain improvement of Pleurotus cornucopiae through the protoplast technique.

关 键 词：姬菇 菌丝体 原生质体 再生 形成 

分 类 号：Q939.53[生物学—微生物学]