缺血后处理减轻大鼠局灶性脑缺血再灌注损伤最佳方案  

Optimal protocol of ischemic postconditioning against focal cerebral ischemia-reperfusion injury in rats

作　　者：孙静[1] 董海龙[1] 栾琪[1] 赵翚[1] 路志红[1] 熊利泽[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2007年第24期2214-2216,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30471664)

摘　　要：目的:探寻缺血后处理(IP)对大鼠局灶性脑缺血损伤保护作用的最佳时间,比较该处理方案与缺血预处理(IPC)脑保护效应.方法:IP最佳时间确定实验分组:60只雄性SD大鼠,随机分为6组(n=10).对照组,行单纯缺血再灌注;IP-15s,30s,1min,2min和5min不同时间点组,分别于大脑中动脉线栓阻闭(MCAO)90min后,再灌注15s,30s,1min,2min和5min,缺血15s,30s,1min,2min和5min,反复三次,组间比较得出IP最佳时间.比较IP最佳时间与IPC脑保护效应实验分组:30只雄性SD大鼠,随机分为3组(n=10).对照组,IP-15s组和IPC组,预先阻闭大脑中动脉20min,再灌注24h后行MCAO90min.上述实验90只动物经再灌注24h后进行神经功能障碍评分.取大脑行2,3,5-氯化三苯四唑(TTC)染色确定脑梗死容积百分比,并行统计学分析.结果:再灌注24h后,在IP最佳时间的确定实验中,IP-15s组神经功能评分和脑梗死容积(34.0±4.8)%明显低于对照组[(58.9±12.2)%,P<0.01];在IP与IPC脑保护效应比较实验中,IP-15s和IPC的神经功能评分与对照组相比差异具有统计学意义(P<0.01),但IP-15s脑梗死容积(36.1±10.3)%大于IPC组[(26.8±3.3)%,P<0.01].结论:IP对大鼠局灶性脑缺血损伤保护作用的最佳时间为15s再灌注/缺血,反复三次;IP的脑保护效应弱于IPC.AIM： To investigate the optimal protocol of ischemic postconditioning （IP） against focal cerebral ischemia-reperfusion injury, and compare the effect of IP with that of ischemic precon- ditioning （ IP, C）. METHODS ： To confirm the optimal protocol of IP, 60 male SD rats weighing 290-310 g were randomly assigned to 6 groups （n = 10 each）： control group and IP groups with different time intervals （15, 30 s, 1,2 and 5 rain）. IP was performed by three cycles of reperfusion/ischemia （R/I） at different time intervals （ 15, 30 s, 1,2 and 5 min respectively） after middle cerebral artery occlusion （MCAO） for 90 rain. To compare the effect of IP with that of IPC, 30 male SD rats weighing 290- 310 g were randomly assigned to 3 groups （n = 10 each） ： control group, IP-15s and IPC. The rats in IP group were subjected to 3 cycles of R/I after 90 rain MCAO at the best time interval（15 s）. The rats in IPC group were subjected to 20 min MCAO 24 h before 90 rain MCAO. The neurological deficit scores （NDS） were evaluated 24 h after reperfusion, and the 90 animals were sacrificed. Infarct volume, as a percentage of volume at normal cerebral hemisphere, was determined by 2, 3, 5-triPhenyltetrazolium （TTC） staining. RESULTS： NDS was significantly lower and the infarct volume was significantly smaller in IP-15 s group [ （34.0 ± 4.8）% ]compared to that of control group[ （58.9 ±12.2）%, P 〈0.01] 24 h after the reperfusion. The NDS in IP-15 s group and IPC group were better than that in control group. The infarct volume in IP-15 s group [ （36. 1± 10.3）% ] was larger than that in IPC group [ （26.8 ± 3.3） %, P 〈 0.01 ]. CONCLUSION ： The optimal protocol of IP to induce ischemic tolerance in brain is three cycles of R/I （15 s/15 s）. And the neuroprotection induced by IPC is stronger than that by IP.

关 键 词：缺血后处理 缺血预处理 再灌注损伤 脑 

分 类 号：R614-33[医药卫生—麻醉学]