子痫前期蜕膜组织差异蛋白的筛选与鉴定  

Screening and identification of differential proteins in deciduas of pre-eclampsia

作　　者：冯亚玲[1] 周昌菊[1] 彭丽秀[1] 

机构地区：[1]中南大学湘雅医学院第三附属医院妇产科,湖南长沙410013

出　　处：《第四军医大学学报》2007年第24期2249-2252,共4页Journal of the Fourth Military Medical University

摘　　要：目的:应用蛋白质组学方法筛选子痫前期差异蛋白,探讨差异蛋白与子痫前期发生的可能关系.方法:收集5例正常足月妊娠妇女和5例足月子痫前期重度患者胎盘母面及宫壁上的蜕膜组织,提取总蛋白.双向凝胶电泳技术对总蛋白进行分离,考马斯亮兰染色,获得蛋白质图谱.用PDQuest软件进行图像分析,寻找差异蛋白,随机选取8个差异蛋白质点进行胶内酶解,用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术获得肽质量指纹谱,搜寻蛋白质数据库鉴定蛋白质并进行初步分析.结果:获得了正常妊娠及子痫前期重度患者蜕膜组织双向电泳凝胶图谱,蛋白质点子痫前期组为(436±13)个点,正常妊娠组为(425±16)个点,图像分析结果显示表达丰度相差2倍以上的差异蛋白质点有14个,它们在子痫前期中均表现下调.随机选取的8个蛋白质点均成功得到鉴定,它们分别是膜联蛋白-Ⅴ,血红蛋白β链,Rho-GDP解离抑制剂1,氯离子细胞内通道蛋白1,原肌球蛋白4,平滑肌蛋白22α2,α1-抗胰蛋白酶和纤维蛋白原β链.结论:子痫前期重度患者蜕膜组织中存在差异蛋白,这些蛋白质在子痫前期的发病过程可能起重要作用.AIM： To screen the differential proteins in deeiduas of pre-eclampsia and to study their roles in pre-eelampsia. METHODS： The deeiduas from 5 normal women of full-term pregnancy and 5 pre-eclampsia patients were collected to obtain the total proteins. The proteins from the 2 groups underwent twodimensional polyaerylamide gel eleetrophoresis （2-D PAGE） and Coomassie brilliant blue stained gel imaging with PDQuest image analysis software. The peptide mass fingerprinting （PMF） was acquired by matrix assisted laser desorption/ionization time-offlight mass spectrometry （MALDI-TOF-MS） . The resulted data were then searched against protein databases for the identification of the proteins, and an initial analysis was made accordingly. RESULTS： 2-DE patterns of the deciduas of normal full-term pregnancy group and preeclampsia group were acquired. According to them, （436 ＋ 13 ） protein spots were seen in preeclampsia group and（ 425 ＋ 16） in normal pregnancy group. Fourteen differ- ential spots were down-regulated in preeclampsia group among them and 8 differential spots were all successfully identified. They were Annexin V, Hemoglobin β chain, Rho-GDP-dissociation inhibitor 1, chloride intracellular channel protein 1 ,Tropomyosin α4 chain, Transgelin 22α2, α1-antitrypsin and Fibrinogen β chain, respectively. CONCLUSION： Differential proteins found in deciduas from patients with severe preeclampsia might play an important role in the pathogenic process of preeclampsia.

关 键 词：先兆子痫 蜕膜 蛋白质组学 电泳 凝胶 双向 

分 类 号：R692.02[医药卫生—泌尿科学]