黄贝平甲胶囊与他巴唑联用对Graves病小鼠细胞因子的影响  

Effects of Huangbei Pingjia capsule and methimazole on cytokines in mice with Graves' disease

作　　者：李晓苗[1] 厚荣荣[1] 李源 谢永宏[3] 陈建宗[4] 冯秀亮[5] 

机构地区：[1]第四军医大学西京医院内分泌科,陕西西安710033 [2]第四军医大学口腔医院麻醉科,陕西西安710033 [3]唐都医院呼吸科,陕西西安710038 [4]第四军医大学西京医院中医药研究中心,陕西西安710033 [5]第四军医大学西京医院实验外科,陕西西安710033

出　　处：《第四军医大学学报》2007年第24期2297-2299,共3页Journal of the Fourth Military Medical University

基　　金：陕西省中医药科学技术研究项目(SXZY07Ab);第四军医大学西京医院临床高新技术资助项目(XJGX0731M29)

摘　　要：目的:观察黄贝平甲胶囊与他巴唑联合应用对毒性弥漫性甲状腺肿(Graves病)小鼠血清中细胞因子水平的影响.方法:用BALB/c雌性小鼠建立Graves病动物模型,在用药物干预后,采用放射免疫分析及夹心ELISA方法检测小鼠血清TT4,TSH,TRAb及细胞因子IL-2,IL-6,TNF-α,IFN-γ水平变化.结果:治疗后黄贝平甲胶囊组小鼠血清中TT4,TRAb水平较模型组明显降低(P<0.05),而TSH水平改变不大,差异无统计学意义;他巴唑组,他巴唑+黄贝平甲胶囊组小鼠血清中TT4,TRAb水平均较模型组显著降低,而TSH水平明显升高(P<0.05);TNF-α,IFN-γ水平变化比模型组显著降低(P<0.05),以联合应用组更为明显,但仍明显高于正常对照组(P<0.05).联合应用组与模型组相比IL-2有所升高(P<0.05);联合应用组与模型组相比IL-6明显降低(P<0.05).结论:黄贝平甲胶囊和他巴唑联合应用对Graves病小鼠血清中细胞因子水平有明显的改善作用,较单独使用他巴唑或黄贝平甲胶囊疗效更为有效.AIM： To observe the effects of Huangbei Pingjia capsule and methimazole on cytokines in mice with Graves＇ disease. METHODS： BALB/c female mouse was used as a Graves＇ disease model. After treatment, TT4, TSH, TRAb, IL-2, IL-6, TNF-α and IFN-γ were measured by radioimmunoassay or sandwich ELISA. RESULTS： Huangbei Pingjia capsule or methimazole alone could not improve the above items of model mice significantly, while, combination of Huangbei Pingjia capsule with methimazole improved them markedly. CONCLUSION： Combination of Huangbei Pingjia capsule with methimazole improves the levels of cytokines in mice with Graves＇ disease significantly and the curative effect is better than methimazole alone.

关 键 词：格雷夫斯病 黄贝平甲胶囊 细胞因子类 

分 类 号：R581.1[医药卫生—内分泌]