RNA干扰c-kit对K562细胞成瘤性的影响  

Effect of siRNA on Expression of c-kit in K562 Cells

作　　者：袁文肃[1] 李克[1] 马丽[1] 芦颖[1] 李庆华[1] 李静[1] 马小彤[1] 庞天翔[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所血液病医院,实验血液学国家重点实验室,300020

出　　处：《天津医药》2007年第12期921-923,I0009,共4页Tianjin Medical Journal

基　　金：天津市应用基础研究计划面上项目(项目编号:05YFJMJC02400)

摘　　要：目的:研究短干扰RNA(siRNA)对K562细胞中c-kit基因表达的影响。方法:构建针对c-kit基因的shRNA表达质粒pSilencer-kit,在脂质体介导下转染人白血病细胞系K562,应用RT-PCR检测c-kit mRNA水平变化,并将转染后的K562细胞注射裸小鼠体内检测成瘤能力的变化。结果:重组体pSilencer-kit转染细胞后显著下调c-kit mRNA水平及其在裸鼠体内成瘤能力,与对照组相比差异有统计学意义(P<0.01)。结论:利用RNA干扰技术能有效抑制靶基因c-kit的表达,为肿瘤的基因治疗提供新思路。Objective： To study the effect of short interference RNA （siRNA） on the expression of c-kit in K562 eeUs. Methods： Plasmid pSileneer-kit expressing c-kit siRNA was constructed. The recombinant plasmid was transfeeted into K562 cells by lipofeetamine.The eeUular c-kit expression was detected by RT-PCR. To analyze the tumorigenic ability, the tumor size in nude rats, which had been injected with K562 and transfeeted K562 eeUs, was measured. Results： The level of c-kit mRNA could be significantly reduced by siRNA. When transfeeted with pSileneer-kit,the expressions of c-kit mRNA were signifieandy inhibited compared with that in control. Conclusion： The vector which express c-kit siRNA was successfully constructed.The RNA interference can effectively inhibit the expression of target gene c-kit,and can be potentially useful in gene therapy of c-kit related cancers.

关 键 词：RNA 小分子干扰 K562细胞 原癌基因蛋白质c—kit 基因表达 转染 逆转录聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学]