穿山龙多糖分离纯化工艺的研究  

Study on Separation and Purification of Dioscorea nipponica Polysaccharide

作　　者：刘莹[1] 戴玉杰[1] 张黎明[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2007年第4期27-30,共4页Journal of Tianjin University of Science & Technology

摘　　要：以粗多糖得率为指标,比较水醇法、水提ZTC吸附澄清剂法和超声波法三种提取方法,并以穿山龙多糖含量为指标,考察采用水醇法提取穿山龙多糖时,回流时间、固液体积比,提取次数对多糖含量的影响;同时,用硫酸-苯酚法对穿山龙粗多糖中的多糖含量进行了测定.实验结果表明,水醇法提取的多糖得率高于水提ZTC吸附澄清剂法和超声波法,分别提高6.0倍和6.6倍.水醇法的最佳提取工艺条件:回流提取时间90,min,液料比1∶10,提取次数3次.The yields of Dioscorea nipponica Polysaccharide extracted by three methods were compared using the yield of crude Polysaccharide as indexes. The three methods were including water-alcohol extraction, ZTC clarifying and ultrasonic extraction. The influences of the circumfluence time, solid-liquid ratio, extraction time on extraction efficiency were investigated using the content of Dioscorea nipponica Polysaccharide as indexes while extracting by water-alcohol extraction. In addition, the content of Dioscorea nipponica Polysaccharide was determined by phenol-sulphuric acid method. The results show that the extraction yield of water-alcohol extraction is significantly higher than ZTC clarifying and ultrasonic wave extraction. The extraction yield of water-alcohol extraction can be improved up to 6.0 times and 6.6 times separately. The optimum technological conditions of water-alcohol extraction are that circumfluence time is 90 min, solid-liquid ratio is 1：10, extraction time is 3.

关 键 词：穿山龙多糖 提取条件 硫酸-苯酚法 ZTC澄清法 水醇法 

分 类 号：R284.2[医药卫生—中药学]