糖杯芳烃基因载体GGC[4]的合成及性能研究  

Synthesis and properties of glycocalixarenes(GGC[4]) as gene carriers

作　　者：田德美[1] 李海兵[1] 

机构地区：[1]华中师范大学化学学院农药与化学生物学教育部重点实验室,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2007年第4期574-577,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(20602015;20772038);湖北省青年杰出人才基金(2007ABB017);武汉市青年晨光计划(200750731283).

摘　　要：设计并制备了一种新的具有分子探针功能的主体分子型非病毒载体双子糖杯芳烃主体分子(GGC[4]),应用透射电子显微镜(TEM)观测到主体分子GGC[4]与DNA分子相互作用形成超分子纳米复合物的微观结构形态.体外细胞转染实验表明其具有较高的转染效率.本研究为设计制备新的非病毒基因载体提供了一种新途径.Non-viral gene vector is one of hot topics of gene therapy. Based on a long time our research on calixarene chemistry and with the progress of artificial virus and gemini surfactant, it is designed to synthesize a new glyco calixarene（GGC[4]） as gene carrier. It is observed that glycocalix[4]arene and DNA are mixed to afford a nano supramolular complexes in TEM images. In vitro transfection assays show that glycocalix [4]arene（GGC[4]） have a high tranfection efficiency. It is very important academic and practice significance for this research to make progress in the field of non-viral gene vector and even gene therapy.

关 键 词：糖杯芳烃 非病毒基因载体 凝胶电泳 透射电子显微镜 

分 类 号：O631.1[理学—高分子化学]