3个编码AP2/EREBP转录因子的基因克隆及其在棉纤维中优势表达的初步分析  

Cloning of three cotton AP2/EREBP genes and their expression preferentially in cotton fibers

作　　者：杜曼丽[1] 倪勇祥[1] 李学宝[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2007年第4期578-582,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金资助项目(30470930);湖北省自然科学基金资助项目(2006ABA181).

摘　　要：从棉花cDNA文库中分离到3个编码AP2/EREBP转录因子的cDNAs序列,命名为GhAP2/EREBP1、GhAP2/EREBP2和GhAP2/EREBP3.利用Real-time RT-PCR对这3个基因表达进行分析,结果表明:GhAP2/EREBP1和GhAP2/EREBP2在棉花纤维中优势表达,而GhAP2/EREBP3在花药中优势表达,在纤维中表达量也较高.这表明上述3个AP2/EREBP转录因子可能在棉花纤维发育过程中起调控作用.AP2/EREBP transcription factors are known to be unique in plants and exist extensively. They are involved in plant growth, development and many physiological and biochemical processes, such as： pathogen and stress resistance, hormone response, floral organogenesis, and so on. Three cDNAs encoding AP2/EREBP transcription factors were isolated from cotton cDNAs libraries. They were defined GhAP2/EREBP1, GhAP2/EREBP2 and GhAP2/EREBP3. Expression analysis of the 3 genes was carried out by quantitative real-time RT-PCR. The results showed that GhAP2/EREBP1 and GhAP2/EREBP2 were expressed highly in fiber, while GhAP2/EREBP3 was expressed highly in anther and at relatively high level in fiber. The results suggest that they may function in fiber development of cotton.

关 键 词：AP2/EREBP转录因子 棉花 基因表达模式 

分 类 号：Q782[生物学—分子生物学]