肿节风浸膏对氧自由基作用的研究  

The Effect of Sarcandra glabra Extracts on Oxygen Free Radical

作　　者：秦俭[1] 王仁生[1] 滕家安[1] 陈健[2] 黄仁彬[2] 

机构地区：[1]广西医科大学第一附属医院,广西南宁530007 [2]广西医科大学,广西南宁530021

出　　处：《时珍国医国药》2007年第12期2945-2946,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西自然科学基金项目(No.桂科学0542090)

摘　　要：目的研究肿节风浸膏对羟自由基(HO.)和超氧阴离子自由基(O2.-)的作用。方法采用邻二氮菲-Fe2+氧化法检测不同浓度肿节风浸膏水溶液对H202/Fe2+体系产生的HO.的清除作用;邻苯三酚自氧化法测定肿节风浸膏溶液对O2.-的作用。结果肿节风浸膏溶液浓度分别为0.2,0.4,0.6,1.2 mg.ml-1时的HO.清除率分别为0.89%,17.52%,49.35%,89.89%,组间有量效关系;肿节风浸膏溶液浓度为0.01 mg.ml-1时对O2.-抑制率为-76.84%。结论肿节风浸膏溶液在体外有显著清除HO.的作用,促进O2.-生成。Objective To study the effect of Sarcandra glabra extracts on superoxide anion free radical （ O2·^- ） and hydroxy free radical （ HO·）. Methods Spectrophotometry （wavelength 322 nm and 510 nm） was used to observe the effects of Sarcandra glabra extracts on superoxide anion free radical and hydroxy free radical. To study scavenging effect of hydroxy free radical in different presence of Sarcandra glabra extracts, we applied 1,10- Phenanthroline- Fe^2＋ oxidative to assay it produced by H2O2/Fe^2＋,and applied autoxidation of pyrogallol to assay superoxide anion free radical, in order to know the effect of Sarcandra glabra extracts on it. Results In the presence of Sarcandra glabra extracts at the concentration of 0.01 mg·ml^-1 in vitro, the generation of O2·^- was inhibited by -76.84%, and in the presence of Sarcandra glabra extracts at the concentration of 0.2,0.4,0.6,1.2 mg·ml^-1 in vitro, the level of HO· was reduced by 0.89% ,17.52% ,49.35% ,89.89%, respectively. It has dose - effect relationship between groups. Conclusion Sarcandra glabra extracts can greatly scavenge HO·, and enhance the production of O2·^-.

关 键 词：肿节风 浸膏 羟自由基 超氧阴离子自由基 

分 类 号：R285.5[医药卫生—中药学]