枫香槲寄生抗肿瘤有效部位总皂苷的提取及含量测定研究  

Studies on the Extraction Technology and Determination of Antitumor Saponins in Viscum liquidambaricolum Hayata

作　　者：杨燕军[1] 沙聪威[2] 安洁[3] 邓永燕[3] 

机构地区：[1]广东省中药研究所广东化工制药职业技术学院,广东广州510520 [2]广东省药物研究所,广东广州510440 [3]广东药学院,广东广州510006

出　　处：《时珍国医国药》2007年第12期2970-2972,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广东省科技计划资助项目(No.2002A301030205);广东省中医药局科研资助课题(No.1040129;1050041)

摘　　要：目的探讨枫香槲寄生抗肿瘤有效部位总皂苷的提取工艺条件及含量测定方法。方法采用正交实验法设计提取条件,以齐墩果酸为对照品,用紫外分光光度法测定总皂苷的含量,优化提取方案。结果回收率为100.3%,RSD=1.54%。各因素对总皂苷提取的影响程度依次为:乙醇浓度>提取次数>提取时间>乙醇倍量。结论提取的最佳工艺条件为5倍量60%乙醇回流提取两次,1.5 h/次,该方法稳定,准确,重现性好。Objective To establish the methods to extract and determine the antitumor saponins from Viscum liquidambaricolum Hayata. Methods The optimized condition for extraction was determined by orthogonal design. UV - Spectrophotometry was used for the determination. Results The recovery ratio was 100.3%, RSD = 1. 54%. The order of factors to affect the total saponins extraction was ethanol concentration 〉 solvent quality 〉 extraction time 〉 extraction times. Conclusion The optimum extraction conditions are 5 times 60% ethanol reflux 2 times, 1.5 hour per time. The methods are stable , accurate and reliable.

关 键 词：枫香槲寄生 紫外可见分光光度法 正交实验 总皂苷 

分 类 号：R284.2[医药卫生—中药学]