大孔树脂对葛根总黄酮的吸附及分离纯化研究  

Research of the Separation and Purification for Flavonoids in Radix Puerarise

作　　者：周媛[1] 程凡[1] 罗少华[1] 

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室,湖北宜昌443002

出　　处：《时珍国医国药》2007年第12期3008-3010,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：以三峡库区丰富的葛根为材料,研究葛根黄酮的分离纯化工艺。方法利用柱层析技术,比较了4种大孔吸附树脂分离纯化葛根总黄酮的效果。结果用AB-8树脂柱进行吸附洗脱,洗脱液为60%乙醇,流速2.4 ml/min,得到的葛根总黄酮(以葛根素计)纯度为53.5%,AB-8树脂的吸附容量为103.2 mg/g。结论建立了AB-8大孔吸附树脂分离纯化葛根总黄酮的适宜条件,发现AB-8大孔吸附树脂在葛根总黄酮的分离纯化中有好的表现。Objective Flavonoids were purified from Radix Puerarise of Yichang. Methods Four excellent macroretiaclar resins were selected for separating flavonoid in Radix Puerarise. Results The extract was absorbed by AB - 8 macroporous absorption resin and was eluted with 60% ethanol at 2.4 ml/min eluting speed. The purity of total flavonoids was 53.5%. The absorptive capacity of AB -8 resin was 103.2% mg/g. Conclusion A feasible technnology was AB -8 for sublimating the total flavonoids in Radix puerariae.

关 键 词：葛根 黄酮 大孔吸附树脂 分离 纯化 

分 类 号：R284.2[医药卫生—中药学]