半枝莲提取物诱导人肝癌SMMC-7721细胞凋亡及其对凋亡相关蛋白表达的影响  

Apoptosis-inducing Effect of Scutellaria Barbata D.Don Extract on Human Liver Cancer SMMC-7721 Cells and the Expression of Apoptosis Related Proteins

作　　者：韦鹏涯[1] 浦洪琴[1] 韦星[1] 宾晓芸[1] 李朝敢[1] 

机构地区：[1]右江民族医学院,广西百色533000

出　　处：《时珍国医国药》2007年第12期3020-3022,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探讨半枝莲提取物(Scutellaria barbata D.Don extract,SBE)对人肝癌SMMC-7721细胞凋亡及凋亡相关蛋白Bcl-2,Survivin和Caspase-3表达的影响。方法体外培养SMMC-7721细胞,以2.5,5和10 mg.L-1SBE处理48 h,并以2.5 mg.L-1顺铂(DDP)作为阳性对照。用MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡率,二步法免疫组化检测Bcl-2,Survivin和Caspase-3蛋白表达。结果与正常对照组相比,SBE各浓度组细胞抑制率显著升高(P(0.001),细胞凋亡率明显上升(P(0.01),Caspase-3蛋白表达显著上升(P<0.01或P<0.05),Bcl-2和Survivin蛋白表达明显下降(P<0.01或P<0.05)。结论SBE具有诱导肝癌SMMC-7721细胞凋亡的作用,其分子机制可能与上调Caspase-3蛋白表达以及下调Bcl-2和Survivin蛋白表达有关。Objective To explore the apoptosis - inducing effect of Scutellaria barbara D. Don extract （SBE） and the expression of apoptosis associated proteins Bcl -2, Survivin and Caspase -3 on human liver cancer SMMC -7721 cells. Methods SMMC - 7721 cells were treated with 2.5, 5 and 10 mg·L^-1 SBE for 48h, and the cells were treated with 2.5mg·L^-1 DDP as positive control. The inhibitory rat was evaluated by MTT assay. The apoptotic rate was determined by flow cytometry. The expression of Bcl - 2, Survivin and Caspase - 3 proteins were detected by two - step immunohistochemical staining. Results Compared with control group, the inhibitory rate was increased obviously（ P 〈 0. 001 ）, the apoptotic rate was increased markedly （P 〈 0.01 ）, the expression of Caspase - 3 protein was increased significantly（ P 〈 0.01 or P 〈 0.05 ）, while Bcl - 2 and Survivin proteins were decreased markedly （ P 〈 0.01 or P 〈 0.05 ） in SBE groups. Conclusion SBE can induce apoptosis of liver cancer SMMC - 7721 cells. The molecular mechanism might be related to up - regulating the expression of Caspase - 3 and down - regulating the expression of Bcl -2 and Survivin apoptosis related proteins.

关 键 词：半枝莲 SMMC-7721细胞 细胞凋亡 Bcl-2 Survivin Caspase-3 

分 类 号：R285.5[医药卫生—中药学]