莪术提取物对PDGF诱导的肝星状细胞内Ca^(2+)和PI3-K的影响  

Effect of Rhizoma Curcumaein on Ca^(2+) and PI3-K of HSC induced by PDGF

作　　者：熊振芳[1] 朱清静 杨玲[3] 张赤志[1] 

机构地区：[1]湖北中医学院,2007级博士生湖北武汉430061 [2]武汉市传染病医院 [3]华中科技大学同济医学院附属协和医院

出　　处：《中西医结合肝病杂志》2007年第6期358-360,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省教育厅课题资助(D200516014);湖北省自然科学基金资助(2004ABA236)

摘　　要：目的:探讨莪术提取物药物血清对PDGF诱导的肝星状细胞(HSC)内Ca2+、PI3-K的影响。方法:制备药物血清,不同浓度的莪术提取物药物血清温育肝星状细胞24小时后,PDGF-BB(10ng/ml)刺激24小时,再加入上述浓度的莪术提取物药物血清,3小时后,又加入PDGF-BB(10ng/ml)作用5分钟,然后收集细胞。采用钙指示剂Fura-2/Am测定细胞内Ca2+,免疫印迹化学发光法检测PI3-K的表达。结果:不同浓度的莪术提取物药物血清与同浓度的生理盐水药物血清比较,能显著抑制PDGF诱导的胞内Ca2+浓度升高(P<0.01)。经10%莪术提取物药物血清处理的HSC,其PDGF诱导的PI3-K表达水平较10%生理盐水药物血清组显著降低(P<0.01)。结论:莪术提取物药物血清能抑制PDGF诱导的肝星状细胞内Ca2+内流及PI3-K的表达。Objective： To investigate the role of Rhizoma Curcumaein extract serum （ ERCS ） on the changes of Ca^2＋ and PI3-K inducing by PDGF. Methods： HSC were treated with ERCS for 24 hours, and stimulated with PDGF-BB for 24 hours, and added the same concentration of ERCS, 3 hours later, stimulated with PDGF-BB for 5 minutes, then collected the cells. Intracellular Ca^2＋ concentration was measured with calcium probe. PI3-K was detected with western blotting enhenced chemiluminescent （ECL） method. Results： ERCS could inhibit the increasing of intacellular calcium concentration induced by PDGF-BB （P 〈 0. 01 ） . Treatment of HSC with ERCS resulted in an inhibition of the PDGF-BBinduced expression of PI3-K （P 〈0. 01 ） . Conclusion： ERCS can inhibti intracellular signal transudation of PDGF in HSC to block the biological effect of PDGF.

关 键 词：莪术提取物/药效学 肝纤维化 肝星状细胞 血小板源生长因子 

分 类 号：R285.5[医药卫生—中药学]