红车轴草提取物对小鼠T淋巴细胞体外活化与增殖的影响  

Effect of Trifoliumpratense L.extract on activation and proliferation of mouse T lymphocytes in vitro

作　　者：杨志[1] 曾耀英[1] 黄秀艳[1] 滕菲[1] 李林[1] 宋兵[1] 姚满林[1] 

机构地区：[1]暨南大学组织移植与免疫实验中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2007年第6期537-540,545,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金："973"国家重点基础研究项目(2006CB504201和2004CB720100)

摘　　要：目的:探讨红车轴草提取物(Trifoliumpratense Leguminosaeextract,TLE)对小鼠T淋巴细胞的体外活化和增殖的影响。方法:MTT比色法检测TLE对细胞的毒副作用及增殖抑制情况;双色荧光抗体染色结合流式细胞术分析TLE对小鼠T淋巴细胞在多克隆刺激剂(ConA)刺激下体外活化抗原CD69表达的影响;羧基荧光素乙酰乙酸(carboxyfluorescein diacetate,succinimidyl ester,CFDA-SE)标记技术结合流式细胞术,分析CD3+T淋巴细胞增殖相关指数。结果:TLE对小鼠淋巴结来源的淋巴细胞微毒副作用;终质量浓度为10、20、30、40 mg/L的TLE对ConA刺激下的T淋巴细胞体外活化抗原CD69表达具有明显的抑制作用(P<0.01),40 mg/L抑制达到峰值;MTT法和CFDA-SE染色法都显示,10、20、30、40 mg/LTLE对ConA诱导的T淋巴细胞增殖具有显著的抑制作用(P<0.01)。结论:TLE对小鼠T淋巴细胞的体外活化和增殖具有明显的抑制作用。Aim：To study the effect of Trifoliumpratense Leguminosae extract（TLE） on activation and proliferation of mouse T lymphocytes in vitro. Methods： The effect of TLE on the side-effect and proliferation of the murine lymphocytes were estimated by MTT test;Double-fluorescent antibody staining plus flow cytometrics were used to detect CD69 expression;CFDA-SE staining CD3^＋ T lymphocytes proliferation. Results： phocytes at the mass concentration of 10.20 plus flow cytometrics to analyze d proliferation of mouse T lymlittle side-effect on mouse lymphocytes. Conclusion： TLE can inhibit the activation and proliferation of mouse T lymphocytes in vitro

关 键 词：红车轴草提取物 T淋巴细胞 活化 增殖 

分 类 号：R392.12[医药卫生—免疫学]