脂肪来源的间充质干细胞分离方法的改进  

The improvement of the extraction method for adipose tissue derived mesenchymal stem cells

作　　者：李冬艳[1] 宇丽[1] 李发涛[1] 刘红[1] 罗京资[1] 

机构地区：[1]暨南大学医学院生物化学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2007年第6期585-589,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：教育部科学技术研究重点基金项目(02910);广东省自然科学基金项目(31875);广州市科委科技攻关重大项目(2002Z1-E0032)

摘　　要：目的:从供体年龄、取材部位、提取方法3方面来研究稳定的脂肪间充质干细胞(ADMSCs)分离提取方法。方法:从兔颈背部皮下、肌间以及腹股沟皮下的脂肪组织取材,分别采用一次消化收集法和多次消化收集法分离提取ADMSCs,单层传代培养。分别比较不同提取方法,老年兔、壮年兔与幼年兔,同一供体不同部位的脂肪组织,所分离的ADMSCs产量和生物学特性。结果:运用多次消化收集法能从脂肪组织中稳定分离出生长旺盛的ADM-SCs,与一次消化收集法相比能显著提高所获取的的细胞产量。从老年兔、壮年兔、幼年兔以及同一供体的不同部位脂肪组织中所提取的ADMSCs生长增殖活性无明显差异,但从单位体积脂肪组织提取得到的ADMSCs产量显著不同。结论:多次消化收集法能显著提高分离获得的ADMSCs产量,是对传统的一次消化收集法的改进。选择不同供体年龄和取材部位提取ADMSCs,对细胞的生物学活性影响不大,但对细胞产量有明显影响。Aim： In order to establish a stable extraction method for adipose tissue derived mesenchyreal stem cells （ADMSCs）, the research was carried out on three respects： donor age, location of fat obtained and extraction way. Methods： Adipose tissue from rabbit was drawn under scruff fur, among muscles, and in groin. ADMSCs were extracted with the single-digestion-separate method or the multi-digestion-separate method. The yield of extracted ADMSCs and the biological activity of cultured ceils were compared among the groups with different extraction methods, from different locations and ages. Results： The eugonic ADMSCs could be stably extracted from adipose tissue with the multi-digestion-separate method, and the yield from it was obviously better than that from the group with the single-digestion-separate method. The proliferation activity of extracted ADMSCs was similar among the rabbits from different ages and locations, but the ADMSCs yield from adipose tissue of unit volume was different significantly. Conclusion：The multi-digestion-separate-method can significantly raise the yield of extracted ADMSCs, which is better than traditional single-digestion-separate-method. The age of rabbit and location of adipose tissue had little effect on the biological activity of extracted ADMSCs, but have obvious effect on the cell yield.

关 键 词：脂肪间充质干细胞 原代细胞 细胞分离提取 组织工程 

分 类 号：R331.1[医药卫生—人体生理学]