利用ARP-PCR法早期鉴定百合远缘杂种  

ARP-PCR identification of hybrids derieved from interspecific hybridization in Lilium

作　　者：柴卫淑[1] 屈云慧[2] 谭学林[1] 熊丽[2] 

机构地区：[1]云南农业大学生物技术与应用学院,云南昆明650201 [2]云南省农业科学院花卉研究所,云南昆明650205

出　　处：《西南农业学报》2007年第6期1296-1298,共3页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金(2003C0028Q)

摘　　要：以不同杂种系的百合品种进行远缘杂交培育杂种。杂种采用Anchored random primer-polymerase chain reaction(ARP-PCR)鉴定,首次报道了利用ARP-PCR技术早期鉴定百合杂种。即利用已经在百合染色体上锚定的引物,扩增亲本和杂种的DNA,鉴定杂种。实验结果表明:ARP-PCR技术中锚定引物的长度是15 bp,退火温度54℃,只扩增出材料的主带型,可重复性高,可靠性好。Interspecific crosses were made to produce hybrids in Lilium. Anchored random primer-polymerase chain reaction （ARP-PCR） identified the hybrids. DNA of the hybrids and their parents was extracted and amplified with ARP-PCR to identify hybrids. The results showed that ARP-PCR was reproductive and reliable technique for Lilium hybrid identification, which could be the first report of hybrid identification in Lilium with PCR markers . In the ARP-PCR,a single primer of 15bp was used in the PCR reaction,with annealing temperature at 54℃.

关 键 词：ARP—PCR百合 远缘杂种 早期鉴定 

分 类 号：S644.1[农业科学—蔬菜学]