龙牙百合茎尖脱毒快繁及种球培育技术  

Studies on in Vitro Virus-free Propagation by Soot-tip and Bulb Culture of Longya Lilium

作　　者：江洪如[1] 余发新[1] 朱祺[1] 高柱[1] 刘腾云[1] 王碧琴[1] 周华[1] 王小玲[1] 

机构地区：[1]江西省科学院生物资源研究所,江西南昌330029

出　　处：《江西农业大学学报》2007年第6期953-956,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家科技部农业科技成果转化资金项目(2004360120581)

摘　　要：当龙牙百合鳞茎在58℃下处理10-20min，再在40℃下处理72h；茎尖大小为0．2～0.4mm时的诱导成活率达60％～66．7％。继代增殖培养基为MS＋1．0mg／LBA＋0．1mg／LNAA时，其增殖系数是3．36。培养基中4．5％～5．5％的食用糖和60d的培养时间利于鳞茎数量和质量的增加。移栽后的小鳞茎培育8个月可达鲜重22g／个以上的脱毒种球。Longya lilium bullets were first treated at 58 %： for 10 - 20 minutes then at 38 ℃ for 72 h. The survival ratio with shoot - tip of 0.2 - 0.4 mm was 60.0%- 66.7%. The reprogactiving coefficient of bulb was 3.36 when a medium for continual culture was MS ＋ 1.0 mg/L BA ＋0.1 mg/L NAA. The quantity and quality of bullet would be increased with a media added with 4.5% - 5.5% sugar and cultured for 60 days. Each transplanted bulb could become virus - free bullet which weighed more than 22 g after 8 months＇ cultivation.

关 键 词：龙牙百合 茎尖脱毒 快速繁殖 种球培育 

分 类 号：S644.103.6[农业科学—蔬菜学]