两种补血草属植物愈伤组织诱导的比较  

Comparison on callus inducement of two species of Limonium Mill.

作　　者：杨颖丽[1] 范小峰[1] 马旭君[1] 张赛娜[1] 王莱[1] 孙坤[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《兰州大学学报（自然科学版）》2007年第6期29-32,38,共5页Journal of Lanzhou University(Natural Sciences)

基　　金：甘肃省自然科学基金项目(3ZS061-A25-080);2007年甘肃省教育厅研究生导师科研项目(0701-23);甘肃省荒漠化防治重点实验室开放基金项目;西北师范大学2006学生学术资助金资助.

摘　　要：以黄花补血草和大叶补血草无菌苗叶片为外植体,采用不同激素配比诱导愈伤组织,并分化获得不定芽,以建立两种补血草无性系,为植株快繁和细胞悬浮培养提供理论依据.结果表明:以补血草无菌苗叶片为外植体进行愈伤组织诱导和分化,可实现补血草快速繁殖,黄花补血草愈伤组织的诱导和分化较大叶补血草的容易.黄花补血草最适愈伤组织诱导培养基为MS+6- BA(0.3～0.5mg/L)+NAA(1.0～2.0mg/L),最适分化培养基为MS+6-BA(0.3～1.0mg/L)+NAA(0.5～1.0 mg/L);大叶补血草最适愈伤组织诱导培养基为MS+6-BA(0.3～0.5mg/L)+NAA 2.0mg/L,最适分化培养基为MS+6-BA 1.0mg/L+NAA 0.5mg/L.To offer theoretical basis of rapid plant propagation and cell suspensional culture, the leaves of sterile seedlings of Limonium aureum（Linn.） Hill and Limonium gmelinii（Willd.） Kantze were used as explants to induce callus in different phytohormone combinations, and they were differentiated to gain adventitious buds. The results indicated that the rapid propagation of Limonium Mill. could be achieved by using the leaves of sterile seedlings of Limonium Mill. as the explant to induce and differentiate callus and that the inducement and differentiation of callus of L. aureum（L.） Hill are more easier than L. gmelinii（W.） Kantze. In addition, the proper induced medium of callus of L. aureum（L.） Hill was MS ＋ 6- BA（0.3-0.5 mg/L） ＋ NAA（1.0-2.0 mg/L）, the proper differentiated medium was MS ＋ 6-BA（0.3-1.0 mg/L） ＋ NAA（0.5-1.0 mg/L）; the proper induced medium of callus of L. gmelinii （W.） Kantze was MS ＋ 6- BA（0.3-0.5 rag/L） ＋ NAA 2.0 rag/L, and the proper differentiated medium was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 mg/L.

关 键 词：黄花补血草 大叶补血草 愈伤组织 诱导 

分 类 号：Q943.1[生物学—植物学]