黄芪多糖对小鼠树突状细胞前体的体内诱导研究  

Effects of Astragalus Polysaccharide on Differentiation and Maturation of Dendritic Cells in Vivo

作　　者：胡微微[1] 臧国庆[1] 余永胜[1] 

机构地区：[1]上海交通大学附属第六人民医院感染病科,上海200233

出　　处：《中国中医药信息杂志》2007年第7期31-33,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：上海市自然科学基金资助项目(03ZR14066)

摘　　要：目的探讨黄芪多糖(astragalus polysaccharide,APS)在体内是否具有诱导小鼠髓源性树突状细胞(dendritic cell,DC)分化和成熟作用,以阐释APS药理作用机制。方法分别设对照组及APS100、200mg/kg剂量组,腹腔注射,体内诱导小鼠髓源性DC前体和未成熟DC,称量脾重,ELISA检测血清中GM-CSF表达量,倒置显微镜观察细胞生长状态,电镜检测细胞形态,流式细胞仪检测CD11c、MHC-Ⅱ类分子及协同刺激分子CD80、CD86表达水平。结果APS注射7d后,APS100、200mg/kg组小鼠脾脏重量均较对照组有明显增加(P<0.01),骨髓细胞体外分离并经rmGM-CSF、rmIL-4诱导培养后,流式细胞检测结果显示:APS100、200mg/kg组CD11c、MHC-Ⅱ类分子表达水平高于对照组(P<0.05),但CD80、CD86表达量较对照组无明显差异;血清中GM-CSF表达量,结果与对照组无明显差异。结论APS能诱导小鼠髓源性DC前体分化为DC,并可能有促进DC成熟的作用,但此作用并非通过刺激机体GM-CSF细胞因子的分泌而发挥作用。Objective To investigate the effect of astragalus polysaccharide, a component of an aqueous extract of Astragalus Membranaceus roots, on differentiation and maturation of dendritic cells in vitro. Methods 30 BALB/c mice were randomly divided into three groups, normal control group, 100, 200 mg/kg APS intraperitoneal injection groups. After one week, weight the mouse spleen, account the splenetic index. Collect the mouse bone marrow cell, induced and cultured with rmGM-CSF and rmIL-4. With inverted microscope to investigate the morphous of DC cell. The phenotypes of DC were detected by flow cytometry and the expression of the GM-CSF protein in serum was tested by ELISA, Results Astragalus polysaccharide injection had obvious effects on the spleen weight of mice, The degree of CD1 lc and MHC-II expression in 100 mg/kg and 200 mg/kg groups on flow cytometry were advanced significantly compared with that in normal control group, but the degree of CDS0 and CD86 was not increased, And the expression of the GM-CSF protein in serum in 100 mg/kg group and 200 mg/kg group were both not increased significantly compared with nomal control group. Conclusion The intraperitoneal injection of astragalus polysaccharide could stimulate the proliferation of the pre DC in bone marrow, The angtigen presentation of DC might be enhanced, but this effects was not positive correlation with concentration of GM-CSF.

关 键 词：树突状细胞 黄芪多糖 小鼠 

分 类 号：R285.5[医药卫生—中药学]