正交试验法优选通络颗粒剂的提取工艺  

Optimization on Extracting Technology of Tongluo Granule by Orthogonal Design

作　　者：鞠海[1] 马秉智[1] 王巍[1] 李淑芳[1] 陆进[1] 

机构地区：[1]中日友好医院药学部,北京100029

出　　处：《中国中医药信息杂志》2007年第7期56-57,79,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的优选通络颗粒剂的提取工艺。方法采用正交试验法,分别对水提部分和醇提部分进行提取工艺考察。水提部分,以提取次数、加水量、提取时间为考察因素,以芍药苷含量和浸出物重量为指标;醇提部分,以乙醇浓度、提取次数、溶剂用量、提取时间为考察因素,以橙皮苷含量和浸出物重量为指标,对提取工艺方案进行优选。结果优选的提取工艺为:水提部分,处方药材用12倍药材量的水煮提3次,每次1.5h;醇提部分,加10倍药材量的60%乙醇回流提取2次,每次1.5h。结论最佳提取工艺的确立为通络颗粒剂的生产提供了理论上的依据。Objevtive To optimize an extracting technology for Tongluo granule by orthogonal design. Water-extracting fraction： with the weight of water-extracting fraction and paeoniflorin content as the indexes, extracting times, water volume and extracting time were screened by L9（3^4） orthogonal test, Alcohol-extracting fraction： with the weight of alcohol-extracting fraction and hesperidin content as the indexes, alcohol concentration, extracting times, alcohol volume and extracting time were screened by L9（3^4） orthogonal test. Results The optimal extraction conditions were as follows： water-extracting fraction： extracting 3 times with 12-fold water, 1.5 hours for each time： alcohol-extracting fraction： refluxing and extracting 2 times with 10-fold 60% alcohol, 1.5 hours for each time. Conclusion The results can provide theoretical basis for production of Tongluo granule.

关 键 词：通络颗粒剂 芍药苷 橙皮苷 正交试验 

分 类 号：R283.5[医药卫生—中药学]