乙酰胆碱酯酶在尼龙网上的固定化研究  

Study of Immobilization of Acetylcholinesterase on Nylon Net

作　　者：周祖新[1] 丁蕙[1] 郭晓明[1] 王根礼[1] 叶伟林[1] 

机构地区：[1]上海应用技术学院化工系,上海200235

出　　处：《化学世界》2007年第12期751-753,708,760,共5页Chemical World

基　　金：上海市科委科研项目(060Z001)

摘　　要：以尼龙网为载体,分别用硫酸二甲酯和三乙基肟四氟化硼作烷基化试剂使尼龙网活化,然后将尼龙网浸入一定浓度的乙酰胆碱酯酶溶液中,置于4°C条件下进行固定化,获得了较好的固定化效果,酶膜活性分别达到4.2 U/cm2和11.1 U/cm2,固定化效率分别达到24.2%和64.1%,重现性好,稳定性好,5个月后活性无明显下降。The nylon net carrier was activated by （CH3）2S04 or （CH3CH2 ）30BE4 as alkylate reagent. Then the nylon net was soaking in acetylcholinesterase solution at 4℃ for immobilization. The activity of the enzyme membrane achieved 4. 2 U/cm^2 and 11.1 U/cm^2 , respectively, and the efficiency of immobilization was 24.2% and 64.1%. The method has good repeatability the stability. The enzyme activity does not obviously decline in five months.

关 键 词：乙酰胆碱酯酶 固定化 酶片 生物传感器 

分 类 号：Q814.2[生物学—生物工程]