X射线低剂量多次辐射对小鼠外周血淋巴细胞的影响  

Effects of low-dose continuously fractionated X-ray irradiation on murine peripheral blood lymphocytes

作　　者：谢漪[1] 张红[1] 党秉荣[1] 郝冀芳[1] 郭红云 王小虎 

机构地区：[1]中国科学院近代物理研究所 [2]甘肃省肿瘤研究所,兰州730000

出　　处：《辐射研究与辐射工艺学报》2007年第6期368-370,共3页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(10675151);甘肃省重点科学技术研究项目(2GS052-A43-008-02);中国科学院西部之光课题2003(O040602XB0)资助

摘　　要：为了评估低剂量多次辐射对健康机体产生的生物学风险。检测了X射线多次照射对BALB/c小鼠免疫系统的影响。采用X射线全身多次照射,第1天照射0.07 Gy,之后每天照射0.08 Gy共12d,剂量率0.2 Gy/min,累积剂量为0、0.07、0.23、0.39、0.55、0.71、0.87和1.03 Gy时取样。照射24 h后取血,用流式细胞仪检测外周血中免疫细胞周期和死亡的变化。结果表明。外周血淋巴细胞的周期在0.39和1.03 Gy时,被阻滞在G_2/M期,在0.07、0.23、0.71和0.87Gy时,被阻滞在G_0/G_1期;细胞的死亡随着累积剂量的增加而增加,在0.71Gy时达到峰值。经过X射线多次全身照射小鼠后,可引起外周血淋巴细胞周期和死亡比例发生变化,符合线性平方模型,造成其免疫系统一定的损伤。For estimating biological risks from low doses continual irradiation, we investigated the effects of exposure to continuously fractionated X-rays on murine immune system. The BALB/c mice were irradiated with 0.07Gy at the first day and 0.08 Gy/d in the following 12 days at a dose rate of 0.2 Gy/min. The peripheral blood lymphocyte cycle and death were determined by flow cytometry at the cumulative doses of 0, 0.07, 0.23, 0.39, 0.55, 0.71, 0.87 and 1.03 Gy respectively. The results showed that the cycle of peripheral blood lymphocyte was arrested in G0/G1 at cumulative doses of 0.07, 0.23, 0.71 and 0.87 Gy, and in G2/M at cumulative doses of 0.39 and 1.03 Gy; the percentage of death of peripheral blood lymphocyte was ascended with dose increasing, and reached the death peak at cumulative doses of 0.71 Gy. The results suggested that low doses continual X-rays total-body irradiated could result in changes of cellular cycle and death, and some damages to immunocytes, which accorded to linear square model.

关 键 词：X射线多次辐射 外周血淋巴细胞 细胞周期和死亡 小鼠 

分 类 号：Q691[生物学—生物物理学] Q782