番红花红O-肝素钠体系的光谱分析及应用  

Analysis and Application for the Spectra of Safranine O-Heparin Sodium System

作　　者：郭小群[1] 马迪[2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]四川理工学院化学系,四川自贡643000

出　　处：《四川理工学院学报（自然科学版）》2007年第6期91-94,共4页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：四川省教育厅青年基金(编号:2006B051)资助项目

摘　　要：在pH6.0的Britton2Robinson(B-R)缓冲溶液中,番红花红O与肝素钠相互作用,形成缔合物而导致溶液吸收光谱发生变化,并对光谱变化进行了对比研究,体系的共振瑞利散射光谱带落在吸收光谱带上,并且散射峰与吸收峰对应。体系的最大共振散射峰在334nm处,而在335nm对应的吸收最弱。这表明,当大分子聚合物吸收了特定波长的能量时,它的外层电子跃迁到更高能态。并且研究了共存物质的影响,表明此方法选择性好,用于肝素钠注射液效价的测定,RSD小于1%。The interaction of safranine o and heparin sodium was studied by the comparison to the variation of absorption spectra in B-R buffer solution with pH=6.0. The position to the strongest resonance rayleigh scattering （RRS） peak （334nm） was corresponding to that of the weakest absorption （335nm）. It indicates that the out-shell electrons of macromolecule will transition to a higher energy level when the absorption of hv with＇ specified wavelength is taken place. Furthermore, the study for the influence of coexistent substances has shown a satisfied selectivity and result which was achieved in the determination of the potencyof heparin sodium injection with RSD〈1%.

关 键 词：番红花红O 肝素钠 分光光度 RRS 

分 类 号：O657.3[理学—分析化学]