抛光酶的酶活测定及在生产中的应用  

Determination of activity of bio-polishing enzyme and its application

作　　者：陈红梅[1] 陈利丽[1] 

机构地区：[1]浙江传化股份有限公司,浙江杭州311215

出　　处：《印染》2007年第24期38-41,共4页China Dyeing and Finishing

摘　　要：用滤纸法测定抛光酶TF-161的酶活，得酶活测定的最佳条件：最大吸收波长500nm,显色剂DNS用量2mL，沸水浴显色时间5min。将抛光酶TF-161应用于棉织物的抛光加工，确定最优工艺为：抛光酶用量1％～3％（owf），工作液pH值4．8左右，温度50～55℃，时间30～60min，可得到理想的抛光效果。The activity of bio-polishing enzyme TF-161was determined by filter paper method, The optimal conditions were as follows： the maximum absorption wavelength was 500nm; the dosage of developer DNS was 2mL and the reaction time in the boiling water was 5 min, At the same time, the bio-polishing enzyme TF-161 was applied in the polishing process of cotton fabric, in which the dosage of bio-polishing enzyme was 1% -3% （owf） with pH value about 4.8, temperature .50 -55 ℃ and time 30 - 60 min, The perfect polishing result could be obtained in this way.

关 键 词：测试 活性 抛光酶 应用 

分 类 号：TS190[轻工技术与工程—纺织化学与染整工程]