肝癌细胞株HepG2的G1期细胞蛋白质组双向电泳图谱的建立  

Development of Two-dimensional Gel Electrophoresis Profiles of Proteome from G1 Phase of HepG2 Cells

作　　者：白瑞霞[1] 王文礼[1] 李丽梅[1] 刘婷[1] 叶纪诚[1] 

机构地区：[1]内蒙古医学院生物化学与分子生物学教研室,呼和浩特010059

出　　处：《科学技术与工程》2008年第1期161-163,共3页Science Technology and Engineering

基　　金：内蒙古医学院重大科研项目(ny2004-zd-002)资助

摘　　要：采用双向电泳分离肝癌细胞株HepG2的G1期细胞的蛋白质,经考马斯亮蓝染色及图像扫描与分析显示:在pH3—10,13cm的IPG胶条上,可分离到227个重复性及分辨率均较好的蛋白斑点,蛋白斑点的匹配率为84%。建立了HepG2的G1期细胞蛋白质组双向电泳图谱,为其蛋白质组学的进一步研究奠定了基础。G1 phase of HepG2 cell proteome was separated by 2-D PAGE. After being stained by Coomassie Brilliant Blue then followed by image scanning and analyzsis, 227 protein spots of good repeatability and resolution were detected in the 2-DE profiles on 13 cm IPG strip （pH 3 - 10）of G1 phase of HepG2 cell. A match rate of 84% of the spots between gels was obtained. The 2-DE profiles of proteome from G1 phase of HepG2 cells is established and lay foundations for the proteome research.

关 键 词：双向电泳 HEPG2细胞 蛋白质组 

分 类 号：R349.3[医药卫生—基础医学]