Regeneration and gene transformation systems of Robima pseudoacacia 'Idaho' mediated by Agrobacterium tumefaciens  

作　　者：Li Min Cai Zao Sun De-you Yin Wei-lun Chen Shou-yi Wang Hua-fang 

机构地区：[1]College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, P. R. China [2]Grape Juice Manufactory of Changbai Mountains, Ji＇an 134200, P. R. China [3]Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, P. R. China

出　　处：《Forestry Studies in China》2006年第4期56-62,共7页中国林学（英文版）

摘　　要：Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.

关 键 词：Robinia pseudoacacia ‘Idaho＇ tissue culture ORGANOGENESIS TRANSFORMATION Agrobacterium tumefaciens 

分 类 号：S72[农业科学—林木遗传育种]