香石竹环斑病毒的分子检测  

The molecular detection of Carnation ringspot virus.

作　　者：陈定虎 于顺 

机构地区：[1]秦皇岛动植物检疫局

出　　处：《植物检疫》1997年第5期257-260,共4页Plant Quarantine

摘　　要：试验具体研究了两类不同性质的ｃＤＮＡ探针对香石竹环斑病毒的检测效果。毒源由中国农业大学植物病毒室提供，繁殖于苋色藜Ｃｈｅｎｏｐｏｄｉｕｍａｍａｒａｎｔｉｃｏｌｏｒ上，提纯该病毒，然后抽提其核酸ＲＮＡ，将ＲＮＡ多聚腺苷化后，以Ｏｌｉｇｏ（ｄＴ）为引物，反转录合成ｃＤＮＡ，补平后与载体连接，转化大肠杆菌，获得阳性克隆，用３２Ｐ和光敏生物素分别标记ｃＤＮＡ制作探针，与ＣＲＳＶ及其ＲＮＡ进行点杂交，结果表明，两类不同性质的探针在检测ＲＮＡ水平上具有相同的灵敏度，其检测极限均达到１ｎｇ／ｍｌ，并对二类探针进行了比较。The artical focuses on the detection of CRSV on molecular level by studying two different kinds of cDNA probe. Through nucleic acid blot hybridization, we can detect CRSV on the molecular level. This method is much more sensitive and rapider than traditional methods. The two different kinds of cDNA probe have the same sensitive in the detection of CRSV RNA, both limited detection level is 1ng/ml, but photobiotin cDNA probe is much safer to human body than isotope cDNA probe, so photobiotin cDNA probe is a very useful tool in the detection of viruses in the port quarantine practice.

关 键 词：香石竹环斑病毒 CDNA 光敏生物素 同位素 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]